Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Jul 1, 2026

Multi-electrode Array Recordings of Human Epileptic Postoperative Cortical Tissue
13:14

Multi-electrode Array Recordings of Human Epileptic Postoperative Cortical Tissue

Published on: October 26, 2014

Perineuronal net abnormalities in epileptic human tissue.

Dalia De Santis1, Cinzia Cagnoli1, Dalila Biancheri1

  • 1Epilepsy Unit, Fondazione IRCCS Istituto Neurologico Carlo Besta, Milan, Italy.

Epilepsia
|June 30, 2026
PubMed
Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Slow-Wave Sleep Fragmentation in Disorders of Arousal and Sleep-Related Hypermotor Epilepsy: A Multicenter Polysomnographic Study.

Neurology·2026
Same author

Pediatric epilepsy surgery: Global survey of invasive explorations.

Epilepsia·2026
Same author

Decoding polymorphous low-grade neuroepithelial tumor of the young (PLNTY): Electroclinical features and molecular signatures in epilepsy surgery candidates.

Epilepsia·2026
Same author

On-site exposure to clinical epilepsy practice for experimental scientists engaged in epilepsy research: A pilot study by the ILAE commission on neurobiology.

Epilepsia open·2026
Same author

Corpora amylacea and glymphatic system in temporal lobe epilepsy patients: Clinicopathological correlation.

Epilepsia·2026
Same author

ILAE-YES global webinar series: Integrating clinical and basic science in epilepsy research.

Epilepsia open·2026
Same journal

Semiology of functional/dissociative seizures in idiopathic generalized epilepsy: An inpatient video-electroencephalographic study.

Epilepsia·2026
Same journal

Cortical stimulation reveals effective disconnection of the epileptogenic network at seizure onset.

Epilepsia·2026
Same journal

Anterior cingulate cortex neuron subtypes differentially regulate seizures.

Epilepsia·2026
Same journal

Ultrafast oscillations in the human brain and their functional significance.

Epilepsia·2026
Same journal

Magnetoencephalographic source imaging improves localization of the epileptogenic zone in multimodal imaging evaluation.

Epilepsia·2026
Same journal

Intermittent fasting with medium-chain triglycerides in drug-resistant epilepsy: A pilot crossover trial.

Epilepsia·2026
See all related articles
This summary is machine-generated.

Perineuronal nets (PNNs) show altered aggrecan expression in epilepsy, with loss in cryptogenic cases and abnormal patterns in focal cortical dysplasia (FCDII). These changes suggest PNN remodeling contributes to epileptogenesis.

Area of Science:

  • Neuroscience
  • Extracellular Matrix Biology
  • Epilepsy Research

Background:

  • Perineuronal nets (PNNs), composed mainly of aggrecan, are crucial for nervous system plasticity and neuron protection.
  • PNNs ensheath parvalbumin (PV) interneurons and are implicated in synaptic regulation.
  • Understanding PNN alterations in epilepsy is vital for elucidating disease mechanisms.

Purpose of the Study:

  • To investigate aggrecan expression within PNNs in human epilepsy specimens.
  • To compare PNN alterations in cryptogenic epilepsy and focal cortical dysplasia type II (FCDII).
  • To explore the role of PNN remodeling in the epileptogenic process.

Main Methods:

  • Analysis of human epilepsy surgical tissues (cryptogenic epilepsy and FCDII) and autoptic controls.
Keywords:
aggrecanepilepsy surgeryfocal cortical dysplasiahuman cortexperineuronal nets

More Related Videos

Preparation of Acute Human Hippocampal Slices for Electrophysiological Recordings
07:31

Preparation of Acute Human Hippocampal Slices for Electrophysiological Recordings

Published on: May 7, 2020

Network Analysis of Foramen Ovale Electrode Recordings in Drug-resistant Temporal Lobe Epilepsy Patients
09:32

Network Analysis of Foramen Ovale Electrode Recordings in Drug-resistant Temporal Lobe Epilepsy Patients

Published on: December 18, 2016

Related Experiment Videos

Last Updated: Jul 1, 2026

Multi-electrode Array Recordings of Human Epileptic Postoperative Cortical Tissue
13:14

Multi-electrode Array Recordings of Human Epileptic Postoperative Cortical Tissue

Published on: October 26, 2014

Preparation of Acute Human Hippocampal Slices for Electrophysiological Recordings
07:31

Preparation of Acute Human Hippocampal Slices for Electrophysiological Recordings

Published on: May 7, 2020

Network Analysis of Foramen Ovale Electrode Recordings in Drug-resistant Temporal Lobe Epilepsy Patients
09:32

Network Analysis of Foramen Ovale Electrode Recordings in Drug-resistant Temporal Lobe Epilepsy Patients

Published on: December 18, 2016

  • Immunohistochemistry and confocal microscopy to assess aggrecan distribution.
  • RNAscope technique to confirm aggrecan production by pathological neurons.

    • Main Results:

    • Control cortices exhibited a clear aggrecan expression gradient, lost in cryptogenic epilepsy with significant aggrecan loss.
    • FCDII showed altered aggrecan labeling in lesions and PNNs around dysmorphic neurons (DNs) with aberrant synaptic formations.
    • Perilesional FCDII cortex and cryptogenic cases displayed marked aggrecan loss, while DNs produced aggrecan in FCDII.

    Conclusions:

    • Etiology-specific PNN remodeling is integral to epileptogenesis.
    • Altered PNNs in FCDII suggest a pathogenic role for DNs within the lesion.
    • Aggrecan loss in PNNs of cryptogenic and FCDII perilesional areas may be seizure-activity mediated.