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Using Solution NMR to Characterize Biomolecular Condensates Under Biphasic Conditions
Published on: April 17, 2026
Biomolecular condensates for proteostasis and potential therapeutic applications
1State Key Laboratory of Membrane Biology, Frontier Research Center for Biological Structure, School of Life Sciences, Tsinghua University, Tsinghua-Peking Center for Life Sciences, Beijing 100084, China.
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Proteostasis is essential for cellular function, and its dysregulation underlies a wide spectrum of diseases. Growing evidence underscores liquid-liquid phase separation (LLPS) as a central mechanism governing protein degradation through the formation of condensates for proteostasis. These membraneless biomolecular condensates concentrate or sequester key degradation factors, substrates, and enzymes, enabling spatiotemporally regulated protein clearance. Condensates for proteostasis represent a mechanism for degrading pathogenic proteins that remain refractory to conventional therapeutics. In this perspective, we explore how LLPS drives the assembly of condensates for proteostasis, outline their physiological functions, and highlight their emerging utility as a versatile platform for targeted protein degradation. Harnessing these condensates offers a promising route to eliminate "undruggable" targets and reestablish proteostasis, opening new avenues for precision medicine across a range of major diseases.
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