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Canonical Wnt Signaling Pathway

Canonical Wnt Signaling Pathway

The gene encoding the main signaling molecules of the Wnt signaling pathways (the Wnt proteins) was discovered almost four decades ago by Nüsslein-Volhard and Wieschaus. They identified and originally named the gene "wingless" (wg) after a phenotype discovered during their landmark genetic screen in Drosophila for body pattern defects. At around the same time, another researcher named Harold Varmus found that a murine tumor virus activates the mammalian wg homolog, Int-1, which results in tumor...

Canonical Wnt Signaling Pathway

Canonical Wnt Signaling Pathway

The gene encoding the main signaling molecules of the Wnt signaling pathways (the Wnt proteins) was discovered almost four decades ago by Nüsslein-Volhard and Wieschaus. They identified and originally named the gene "wingless" (wg) after a phenotype discovered during their landmark genetic screen in Drosophila for body pattern defects. At around the same time, another researcher named Harold Varmus found that a murine tumor virus activates the mammalian wg homolog, Int-1, which results in tumor...

Non-Canonical Wnt Signaling Pathways

Non-Canonical Wnt Signaling Pathways

Wnt is a zygotic effect gene that is expressed during very early embryonic development. It regulates various processes in animals starting from early development through the adult stage, such as organogenesis in the embryo and maintenance of neuronal and blood stem cells. Wnt proteins can induce a wide variety of intracellular pathways depending upon the specific abilities of different Wnt ligands to form a complex with shared and cognate receptors in the presence of different co-receptors. The...

Role of Ephrin-Eph Signalling in Intestinal Stem Cell Renewal

Role of Ephrin-Eph Signalling in Intestinal Stem Cell Renewal

Erythropoietin-producing hepatocellular carcinoma receptor (Eph) and its ligand, Eph receptor-interacting protein (Ephrin) were first discovered in the human carcinoma cell line, hence the name. Ephrin-Eph interaction guides cells to reach their appropriate location in adult tissues. They also play an essential role in the immune system by helping in immune cell migration, adhesion, and activation. Based on their structure and function, Eph is divided into two classes — EphA and EphB.

Hedgehog Signaling Pathway

Hedgehog Signaling Pathway

The Hedgehog gene (Hh) was first discovered due to its control of the growth of disorganized, hair-like bristles phenotype in Drosophila, much like hedgehog spines. Hh plays a crucial role in the development of organs and the maintenance of homeostasis in both invertebrates and vertebrates. However, while Drosophila has only one Hh protein, mammals have multiple functional Hedgehog proteins - Sonic (Shh), Desert (Dhh), and Indian Hedgehog (Ihh). All of these homologous proteins have adapted to...

TGF - β Signaling Pathway

TGF - β Signaling Pathway

The TGF-β signaling pathway regulates cell growth, differentiation, adhesion, motility, and development. TGF-β ligands that induce TGF-β signaling are synthesized in their latent form. Several proteases or cell surface receptors such as integrins act upon the latent form, releasing the active ligand. There are three types of mammalian TGF-βs: (TGF-β1, TGF-β2, and TGF-β3) that bind as homodimers or heterodimers to TGF-β receptors. The TGF-β receptors are of three kinds RI, RII, and RIII. The RI...

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Related Experiment Video

Updated: Jul 2, 2026

Studying Wnt Signaling During Patterning of Conducting Airways

Studying Wnt Signaling During Patterning of Conducting Airways

Published on: October 16, 2016

Secreted Frizzled-Related Protein 1 Controls Distal Lung Formation via Wnt and PDGF Signaling.

Nooralam A Rai¹, Tina Zelonina², Kiran Chada³

¹Department of Pediatrics, Columbia University Medical Center, New York, NY.

Developmental Biology

|June 30, 2026

Summary

Secreted frizzled-related protein 1 (SFRP1) is crucial for normal lung development. Its absence disrupts lung structure and fibroblast signaling, highlighting its role in neonatal lung diseases.

Keywords:

Wnt signaling pathway fibroblasts lung development

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Related Experiment Videos

Last Updated: Jul 2, 2026

Studying Wnt Signaling During Patterning of Conducting Airways

Studying Wnt Signaling During Patterning of Conducting Airways

Published on: October 16, 2016

Modeling Paracrine Noncanonical Wnt Signaling In Vitro

Modeling Paracrine Noncanonical Wnt Signaling In Vitro

Published on: December 10, 2021

The Soft Agar Colony Formation Assay

The Soft Agar Colony Formation Assay

Published on: October 27, 2014

Area of Science:

Developmental Biology
Pulmonology
Molecular Biology

Background:

Normal lung development relies on mesenchymal signaling for structural maturation and cell differentiation.
The role of Wnt modulators like secreted frizzled-related protein 1 (SFRP1) in lung development is not fully understood.
Understanding SFRP1's function could identify therapeutic targets for neonatal lung diseases.

Purpose of the Study:

To investigate the role of SFRP1 in distal lung development and mesenchymal signaling using a genetic knockout model.

Main Methods:

Compared lung development in Sfrp1 knockout (Sfrp1-/-) and wild-type (Sfrp1+/+) mice using histology and morphometry.
Utilized single-cell RNA sequencing and in situ hybridization to determine cell-specific SFRP1 expression.
Performed bulk RNA sequencing and pathway analysis on mesenchymal cells during the saccular stage.

Main Results:

SFRP1 ablation resulted in abnormal lung morphometry across developmental stages (canalicular, saccular, alveolar).
SFRP1 is expressed in mesenchymal cells, primarily fibroblasts, during early lung development.
Significant alterations in signaling pathways were observed in whole lung and specific cell populations (PDGFRα+) in the saccular stage.

Conclusions:

Wnt signaling, mediated by SFRP1, regulates lung sacculation and alveologenesis via fibroblast signaling and differentiation.
These findings link Wnt signaling in lung development to conditions like bronchopulmonary dysplasia (BPD).
SFRP1 plays a role in fibroblast differentiation, potentially after lung injury.