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Updated: Jul 2, 2026

A Method for Lineage Tracing of Corneal Cells Using Multi-color Fluorescent Reporter Mice
Published on: December 18, 2015
Lineage tracing from cellular heritage to disease destiny.
Huan Zhu1, Xiuxiu Liu1, Muxue Tang1
1CAS CEMCS-CUHK Joint Laboratory, New Cornerstone Science Laboratory, Key Laboratory of Multi-Cell Systems, Shanghai Institute of Biochemistry and Cell Biology, Center for Excellence in Molecular Cell Science, Chinese Academy of Sciences, University of Chinese Academy of Sciences, Shanghai, China.
Cell lineage tracing reconstructs cellular ancestry to understand how development impacts cell identity and disease. New tools and multi-omics integration will advance precision medicine by decoding cell history.
Area of Science:
- Cell Biology
- Genetics
- Developmental Biology
Background:
- Cellular identity and function are determined by developmental history.
- Cell lineage tracing is crucial for understanding cell fate in health and disease.
- Recent technological advancements have improved in vivo lineage reconstruction.
Purpose of the Study:
- To review recent tools for cell lineage tracing.
- To highlight the application of these tools in pathological conditions.
- To discuss future directions integrating lineage tracing with multi-omics.
Main Methods:
- Recombinase systems
- Synthetic barcoding
- Natural barcoding approaches
- In vivo cell lineage tracing
Main Results:
- Advanced tools enable high-resolution reconstruction of cellular ancestries.
- These tools facilitate investigation of pathological lineage programs.
- Integration with single-cell multi-omics is a promising future direction.
Conclusions:
- Cell lineage tracing is key to understanding how cell history shapes current state and future potential.
- New tools and approaches are advancing the study of diseases like cancer and cardiovascular disease.
- This field is paving the way for a new era of precision medicine.
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