Simulated Natural Nanoparticles in Bai-Hu-Tang Decoction: Preparation, Characterization, Pharmacokinetics, and...

Objective: The purpose of this study was to prepare a simulated form of N-BHT (Simu-N-BHT) using a counter-evidence method and to analyze the mechanism by which a natural nanophase was formed.

The morphology of nanoparticles in each group was observed by transmission electron microscopy. The Simu-N-BHT nanoparticles were prepared by the decocting-dialysis method. The study also characterized Simu-N-BHT nanoparticles, in vitro release, in vivo pharmacokinetics, antipyretic effects, and pathological analysis.

Through the TEM images and correlation analysis of 15 groups, it can be seen that the formation of nano-colloidal particles is inseparable from japonica rice. The Simu-N-BHT nanoparticles were very similar to N-BHT nanoparticles in terms of morphology and were spherical, with a mean size of 223 ± 11.25 nm. Furthermore, Simu-N-BHT nanoparticles exhibited a crystal structure with specific thermodynamic properties. The pharmacokinetic behavior of these nanoparticles supports the non-compartmental model and exhibits antipyretic effects similar to those of N-BHT nanoparticles. The pathological results indicate that treatment with Simu-N-BHT can alleviate lung injury in rats.

The nano-phase solution obtained through modern pharmaceutical methods exhibits results similar to the naturally formed N-BHT in decoctions in terms of appearance, particle size, and thermodynamic properties. This provides new methods and experimental evidence for the research and development of novel traditional Chinese medicine preparations.

This study ultimately provides a natural polymer carrier material-amylopectin derived from japonica rice-for the formation of spherical nanoparticles with smooth surface morphology.