Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Jul 2, 2026

Radionuclide-fluorescence Reporter Gene Imaging to Track Tumor Progression in Rodent Tumor Models
10:04

Radionuclide-fluorescence Reporter Gene Imaging to Track Tumor Progression in Rodent Tumor Models

Published on: March 13, 2018

Drug Screening of Nitroreductase Modulators Using an Atomic-Engineered Activatable Fluorescent Probe for Tumor

Fei Han1, Suntao Shi1, Liangying Mi1

  • 1State Key Laboratory of Natural Product Chemistry, College of Chemistry and Chemical Engineering, Lanzhou University, Lanzhou 730000, China.

Journal of Medicinal Chemistry
|July 1, 2026
PubMed
Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Easy Access to <i>N,N</i>-Disubstituted 2-Imidazolidinones from Weinreb-Type Amides and Enamides via Visible-Light-Mediated Radical Smiles Rearrangement.

Organic letters·2026
Same author

Emergence of a Potent AChE Inhibitor with Antioxidant and Neuroprotection Abilities.

ACS medicinal chemistry letters·2026
Same author

Tuning Lipophilicity to Develop an MMP-Independent Fluorescent Probe for Visualizing Mitochondrial Peroxynitrite in Drug-Induced Liver Injury.

Analytical chemistry·2026
Same author

A self-assembled fluorescent probe for H<sub>2</sub>O<sub>2</sub> detection in NAFLD diagnosis.

Organic & biomolecular chemistry·2025
Same author

Palladium-catalyzed <i>in vivo</i> bioorthogonal activation of <i>N</i>,<i>N</i>-dialkyl-α-hydroxymethyl acrylamide for efficient payload release.

Journal of materials chemistry. B·2025
Same author

Boosting PDT with DPA-NI-Bu: high photocytotoxicity through redox homeostasis perturbation.

Journal of materials chemistry. B·2025
This summary is machine-generated.

Researchers developed novel nitroreductase (NTR)-activated fluorescent probes for precise tumor imaging. These probes aid in distinguishing cancer cells and show potential for intraoperative navigation and drug screening.

Area of Science:

  • Biomedical Engineering
  • Chemical Biology
  • Molecular Imaging

Background:

  • Nitroreductase (NTR) is a hypoxia-upregulated enzyme and a potential biomarker for solid tumors.
  • Effective biosensors are needed for NTR detection, driving interest in fluorescent probes.
  • Cyanine dyes are versatile fluorophores used in various biomedical applications.

Purpose of the Study:

  • To design and synthesize novel NTR-activated fluorescent probes using atomic engineering.
  • To evaluate the lead probe (HCy-NS-4) for its optical properties and imaging capabilities.
  • To explore the potential of the probe in intraoperative tumor navigation and drug screening.

Main Methods:

  • Atomic engineering via heteroatom substitution (C, O, S) to design four NTR-activated probes.

More Related Videos

Kinetic Screening of Nuclease Activity using Nucleic Acid Probes
06:52

Kinetic Screening of Nuclease Activity using Nucleic Acid Probes

Published on: November 1, 2019

A Bright NIR-II Fluorescence Probe for Vascular and Tumor Imaging
05:51

A Bright NIR-II Fluorescence Probe for Vascular and Tumor Imaging

Published on: March 17, 2023

Related Experiment Videos

Last Updated: Jul 2, 2026

Radionuclide-fluorescence Reporter Gene Imaging to Track Tumor Progression in Rodent Tumor Models
10:04

Radionuclide-fluorescence Reporter Gene Imaging to Track Tumor Progression in Rodent Tumor Models

Published on: March 13, 2018

Kinetic Screening of Nuclease Activity using Nucleic Acid Probes
06:52

Kinetic Screening of Nuclease Activity using Nucleic Acid Probes

Published on: November 1, 2019

A Bright NIR-II Fluorescence Probe for Vascular and Tumor Imaging
05:51

A Bright NIR-II Fluorescence Probe for Vascular and Tumor Imaging

Published on: March 17, 2023

  • Optical characterization of probes, including fluorescence enhancement and emission wavelength.
  • In vitro and in vivo studies for imaging hypoxic NTR, distinguishing cell types, and assessing tumor navigation.

    • Main Results:

    • HCy-NS-4 was identified as the lead probe with near-infrared emission (712 nm) and >18-fold fluorescence enhancement.
    • The probe enabled precise imaging of hypoxic NTR and differentiation of malignant from nonmalignant cells.
    • Luteolin was identified as an NTR modulator with antitumor activity, using HCy-NS-4 as a screening tool.

    Conclusions:

    • The developed NTR-activated probes are effective tools for precise tumor imaging and intraoperative navigation.
    • HCy-NS-4 demonstrates significant potential for distinguishing tumor tissues and guiding surgical procedures.
    • This study presents a novel model for drug screening by combining molecular design with inhibitor discovery.