Related Experiment Video
Updated: Jul 2, 2026
Composition and Properties of Aquafaba: Water Recovered from Commercially Canned Chickpeas
Published on: February 10, 2018
Structural characterization and functional properties of safflower seed protein extracted by natural deep eutectic
Mengmeng Wei1, Chunyan Wang1, Sheng Geng1
1School of Food Science, Henan Institute of Science and Technology, Xinxiang 453003, China.
None:
In this study, betaine-based natural deep eutectic solvents (NADES) were formulated for the extraction of safflower seed protein (SSP). Among the five NADES screened, betaine-glycerol (1:2 M ratio) was identified as optimal, achieving a protein purity of 85.66% and an extraction yield of 56.66%. Using response surface methodology, the extraction yield was further increased to 66.45% ± 0.43% under optimized conditions (29% water content, 52 °C, solid-to-liquid ratio of 1:24). Compared with protein extracted by alkaline solubilization-acid precipitation (AEAP), NADES-extracted SSP exhibited a lighter color, higher α-helix content (23.86%), and lower β-sheet content (34.16%), with better preservation of subunit integrity. Functionally, it demonstrated superior solubility (85.00%), emulsifying activity (93.23 m2/g), and foaming capacity (209.73%) at pH 3.0, along with higher oil-holding capacity. The betaine-glycerol NADES system was therefore established as a green and efficient approach for SSP extraction.
