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A functionalized single-layered titanium carbide MXene based biosensor for selective H2O2 detection using covalently
Devarasu Mohanapriya1, Vadakke Purakkal Sruthi1, Sellappan Senthilkumar1
1Department of Chemistry, School of Advanced Sciences, Vellore Institute of Technology (VIT), Vellore-632014, India. senthilkumar.s@vit.ac.in.
Journal of Materials Chemistry. B
|July 1, 2026
Summary
A novel biosensor was developed using amine-functionalized Ti3C2Tx MXene and horseradish peroxidase for sensitive hydrogen peroxide detection. This advancement offers improved environmental monitoring and biomedical diagnostics.
Area of Science:
- Materials Science: Synthesis and characterization of functionalized MXene materials.
- Electrochemistry: Development of electrochemical biosensors for analyte detection.
- Biotechnology: Immobilization of enzymes for biosensing applications.
Background:
- Hydrogen peroxide (H2O2) is a crucial molecule in biological and environmental systems.
- Accurate H2O2 determination is vital for environmental monitoring and biomedical diagnostics.
- Development of efficient and sensitive biosensors is necessary for H2O2 quantification.
Purpose of the Study:
- To synthesize and characterize an amine-functionalized, single-layered Ti3C2Tx MXene (NH2-S-Ti3C2Tx).
- To construct a novel electrochemical biosensor by immobilizing horseradish peroxidase (HRP) onto the NH2-S-Ti3C2Tx modified glassy carbon electrode (GCE).
- To evaluate the performance of the fabricated HRP/NH2-S-Ti3C2Tx/GCE biosensor for the electrocatalytic detection of H2O2.
Main Methods:
- Synthesis of amine-functionalized Ti3C2Tx MXene.
- Modification of a glassy carbon electrode (GCE) with NH2-S-Ti3C2Tx.
- Covalent immobilization of horseradish peroxidase (HRP) onto the modified electrode using glutaraldehyde.
- Electrochemical characterization and H2O2 detection using cyclic voltammetry and amperometry.
Main Results:
- The fabricated biosensor (HRP/NH2-S-Ti3C2Tx/GCE) exhibited a well-defined redox peak at -0.45 V, characteristic of HRP.
- The biosensor demonstrated a broad linear detection range for H2O2 from 20 µM to 1160 µM.
- A low limit of detection (5.3 µM) and high sensitivity (0.078 µA mM-1 cm-2) were achieved, attributed to direct electron transfer via covalent HRP immobilization.
- The sensor showed good performance in real-time H2O2 detection with satisfactory recovery rates.
Conclusions:
- The developed HRP/NH2-S-Ti3C2Tx/GCE biosensor is highly effective for sensitive and selective H2O2 detection.
- The use of NH2-S-Ti3C2Tx as a platform facilitates efficient enzyme immobilization and direct electron transfer, enhancing biosensor performance.
- This novel biosensor holds significant potential for applications in environmental monitoring and biomedical diagnostics.
