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Updated: Jul 2, 2026

A Protocol for Real-time 3D Single Particle Tracking

A Protocol for Real-time 3D Single Particle Tracking

Published on: January 3, 2018

In situ polymer nanoparticle densitometry via real-time 3D single-particle tracking.

Jacob Reinach¹, Donggeng Yu¹, Kevin D Welsher¹

¹Department of Chemistry, Duke University, Durham, North Carolina 27708, USA.

The Journal of Chemical Physics

|July 1, 2026

Summary

This summary is machine-generated.

A new single-particle technique measures the density and mass of polymer nanoparticles in real-time. This method reveals densely packed monomers within growing polynorbornene nanoparticles, advancing nanoscale chemical analysis.

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Advanced Compositional Analysis of Nanoparticle-polymer Composites Using Direct Fluorescence Imaging

Advanced Compositional Analysis of Nanoparticle-polymer Composites Using Direct Fluorescence Imaging

Published on: July 19, 2016

Area of Science:

Polymer Chemistry
Nanotechnology
Physical Chemistry

Background:

Single-particle techniques offer insights into nanoscale dynamics.
Characterizing evolving chemical systems at the single-particle level requires advanced tools.
Understanding polymer nanoparticle structure is crucial for material science.

Purpose of the Study:

Introduce a novel single-particle method for measuring polymer nanoparticle density and mass.
Apply the technique to ring-opening metathesis polymerization (ROMP) of norbornene.
Enable in situ, real-time characterization of individual nanoparticle properties.

Main Methods:

Utilized 3D single-molecule active real-time tracking (3D-SMART).
Measured nanoparticle descent velocity to determine density.
Analyzed size and density to infer monomer packing.

Main Results:

Successfully measured density and mass of freely diffusing polymer nanoparticles.
Determined polynorbornene nanoparticles have a density of 850 ± 30 kg/m³.
Indicated nanoparticles contain approximately 3.5 × 10⁹ densely packed monomers.

Conclusions:

The developed technique enables in situ characterization of individual growing polymer nanoparticles.
Density and size analysis provide insights into nanoparticle composition and structure.
The method's accuracy is influenced by particle size, solvent, and trajectory data.