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Updated: Jul 2, 2026

A Filtration-based Method of Preparing High-quality Nuclei from Cross-linked Skeletal Muscle for Chromatin Immunoprecipitation
Published on: July 6, 2017
Chromatin dynamics and cellular energy homeostasis
Tamaki Suganuma1, Jerry L Workman1
1Stowers Institute for Medical Research, 1000 E. 50th Street, Kansas City, MO 64110, USA.
Abstract:
Metabolism is a complex consortium of chemical reactions, consuming and releasing energy. In addition, cellular respiration is a pivotal catabolic process of nutrients using oxygen to generate ATP. Dynamics of chromatin structure are fundamental to genomic events in all cellular processes. Therefore, chromatin consumes robust cellular energy formed in metabolism. To regulate cellular processes, chromatin functions and metabolism need to be tightly coordinated in response to environmental changes including nutrition availability. Indeed, molecular oxygen directly impacts DNA and chromatin modification and influences cell fate. Herein, we review fundamentals of chromatin dynamics, nucleosome modifications, and roles of chromatin dynamics in spatiotemporal regulation of cellular energy homeostasis.
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