Related Experiment Video
Updated: Jul 3, 2026
Visualizing Clathrin-mediated Endocytosis of G Protein-coupled Receptors at Single-event Resolution via TIRF Microscopy
Published on: October 20, 2014
Protocol for quantitative live-cell imaging of early GPCR trafficking using acid-stable afCFP-Venus FRET
Yu Guo1, Toshimasa Takahashi2, Cheng Wang1
1Department of Geriatric and General Medicine, Osaka University Graduate School of Medicine, Suita, Osaka 565-0871, Japan.
Abstract:
Here, we present a protocol to visualize and quantify β-arrestin-dependent signaling of the angiotensin II type 1 receptor (AT1R). We describe steps for detecting receptor-β-arrestin interactions during early trafficking events under our imaging conditions using an afCFP-Venus FRET pair. We then detail procedures for visualizing early intracellular signaling dynamics following AT1R activation and for analyzing GPCR trafficking and signaling dynamics in living cells.
