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Updated: Jul 3, 2026

Transnuclear Mice with Pre-defined T Cell Receptor Specificities Against Toxoplasma gondii Obtained Via SCNT
Published on: September 30, 2010
Protocol for somatic cell nuclear transfer in the common marmoset
Yoko Kurotaki1, Yuko Yamada2, Narumi Ogonuki3
1Integrative Developmental Engineering Division, RIKEN BioResource Research Center, Tsukuba, Ibaraki 305-0074, Japan; Department of Development Research Translational Research Division, Central Institute for Experimental Medicine and Life Science, Kawasaki, Kanagawa 210-0821, Japan.
Abstract:
Somatic cell nuclear transfer (SCNT) in non-human primates is highly inefficient, as many reconstructed embryos arrest during preimplantation development. Here, we provide a protocol for performing SCNT in the common marmoset. We describe steps for oocyte collection, in vitro maturation, enucleation, and nuclear transfer using fibroblast or cumulus donors. We then detail procedures for oocyte activation, triple epigenetic enhancement, and embryo culture up to the blastocyst stage. For complete details on the use and execution of this protocol, please refer to Matoba et al.1.
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