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Updated: Jul 3, 2026
08:52
Novel Object Recognition Test for the Investigation of Learning and Memory in Mice
Published on: August 30, 2017
Apelin receptor antagonist (ML221) facilitates memory reconsolidation in novel object recognition task
Yu Xudong1, Luo Susu2, Ye Cuiting2
1The Brain Cognition and Brain Disease Branch, Pu Ai Medical School, Shaoyang University, 422000, Shaoyang, China; College of Food Science and Engineering, Central South University of Forestry and Technology, 410004, Changsha, China.
Physiology & Behavior
|July 1, 2026
Summary
Blocking the apelin receptor (APJ) with ML221 enhances memory reconsolidation in the novel object recognition task. This suggests APJ antagonism may be a therapeutic target for memory enhancement.
Area of Science:
- Neuroscience
- Pharmacology
Background:
- The apelin receptor (APJ) and its ligand apelin are implicated in memory processes.
- The role of APJ blockade in memory reconsolidation is not well understood.
Purpose of the Study:
- To investigate the effect of the selective APJ antagonist ML221 on memory reconsolidation using the novel object recognition (NOR) paradigm.
Main Methods:
- Administration of ML221 at different doses and time points relative to memory reactivation in the NOR task.
- Assessment of memory performance and general locomotor activity.
Main Results:
- ML221 (0.1 and 0.3 mg/kg) administered immediately after reactivation enhanced NOR memory performance.
- This facilitative effect was dose-dependent and time-sensitive, with no effect when administered 6 hours post-reactivation.
- ML221 did not affect locomotor activity, indicating specific effects on memory.
Conclusions:
- APJ antagonism by ML221 enhances novel object recognition memory reconsolidation.
- These findings highlight the potential of targeting the APJ pathway for memory modulation.
