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Related Experiment Video

Updated: Jul 3, 2026

Synthesis of Wavelength-shifting DNA Hybridization Probes by Using Photostable Cyanine Dyes
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Synthesis of Wavelength-shifting DNA Hybridization Probes by Using Photostable Cyanine Dyes

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A General Strategy for Developing Si-Rhodamine-Based Fluorogenic Dyes for Advanced Bioimaging and Biosensing.

Zhengda Chen1, Yajie Shi2, Li Jiang1,2

  • 1School of Biomedical Engineering, Shanghai Jiao Tong University, 800 Dong Chuan Road, Shanghai 200240, China.

Journal of the American Chemical Society
|July 1, 2026
PubMed
Summary

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This summary is machine-generated.

Researchers developed novel fluorogenic Si-rhodamine (SiR) dyes with a molecular rotor. These dyes offer a 3000-fold fluorescence increase for advanced bioimaging and biosensing applications.

Area of Science:

  • Chemical Biology
  • Biophotonics
  • Molecular Imaging

Background:

  • Si-rhodamine (SiR) dyes are crucial for near-infrared (NIR) bioimaging due to their photostability and brightness.
  • Developing SiR derivatives with high fluorogenicity (fluorescence enhancement) is a significant challenge in rational dye design.

Purpose of the Study:

  • To establish a general strategy for creating high-performance fluorogenic SiR derivatives.
  • To develop novel dyes for robust live-cell and in vivo imaging and biosensing.

Main Methods:

  • Incorporation of a molecular rotor at the meso position of the SiR scaffold to create SF dyes.
  • Utilizing a twisted intramolecular charge transfer (TICT) mechanism for fluorescence switching.
  • Conjugating SF dyes with chloroalkane (CA) for HaloTag labeling and applying them in stimulated emission depletion (STED) microscopy.

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Synthesis, Cellular Delivery and In vivo Application of Dendrimer-based pH Sensors
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Synthesis, Cellular Delivery and In vivo Application of Dendrimer-based pH Sensors

Published on: September 10, 2013

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Last Updated: Jul 3, 2026

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Synthesis, Cellular Delivery and In vivo Application of Dendrimer-based pH Sensors
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Main Results:

  • Achieved a maximum fluorescence on/off ratio exceeding 3000-fold via the TICT mechanism.
  • Demonstrated high cellular brightness and photostability of CA-conjugated SFs for HaloTag labeling in live cells and in vivo.
  • Enabled subdiffraction resolution imaging in live cells using STED microscopy.
  • Constructed chemigenetic Ca2+ indicators with large dynamic ranges for sensitive detection of Ca2+ dynamics.

Conclusions:

  • A general design strategy for high-performance NIR fluorogenic dyes (SFs) was established.
  • SF dyes offer significant advancements for advanced bioimaging and biosensing, including Ca2+ dynamics.
  • The developed dyes enable sensitive, high-contrast imaging with subdiffraction resolution in live systems.