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Published on: June 7, 2024
Physiological and transcriptomic analyses of Rosa persica in response to drought stress and functional validation of
Na Li1, Yang Cui1, Yangfeng Liang1
1School of Landscape Architecture, Beijing Forestry University, Beijing Key Laboratory of Ornamental Plants Germplasm Innovation and Molecular Breeding, Beijing Laboratory of Urban and Rural Ecological Environment, Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants of Ministry of Education, State Key Laboratory of Efficient Production of Forest Resources, National Engineering Research Center for Floriculture, Engineering Research Center of Landscape Environment of Ministry of Education, Beijing, China.
Abstract:
Rosa persica exhibits strong drought tolerance and represents a valuable parental resource for stress-resistance breeding in roses. To elucidate the molecular regulatory mechanisms underlying its drought stress responses, drought stress was imposed using a restricted irrigation regime, and drought-responsive traits were systematically investigated at the phenotypic, physiological, and molecular levels. Under drought stress, R. persica displayed leaf chlorosis, a significant reduction in leaf number, and decreased leaf thickness. At the physiological level, drought stress induced a significant accumulation of soluble sugar, proline, and starch, along with increased catalase (CAT) activity. Transcriptome analysis identified numerous differentially expressed genes in response to drought stress. Furthermore, weighted gene co-expression network analysis (WGCNA) identified an RpERF113-like gene from the AP2/ERF family that is closely associated with the drought stress response in R. persica. Functional characterization in Arabidopsis thaliana demonstrated that overexpression of RpERF113-like significantly enhanced drought tolerance by increasing proline content, elevating CAT activity, and reducing malondialdehyde (MDA) content. Collectively, these findings provide new insights into the molecular mechanisms underlying drought responses in R. persica.
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Transcription
Transcription is the process of synthesizing RNA from a DNA sequence by RNA polymerase. It is the first step in producing a protein from a gene sequence. Additionally, many other proteins and regulatory sequences are involved in the proper synthesis of messenger RNA (mRNA). Regulation of transcription is responsible for the differentiation of all the different types of cells and often for the proper cellular response to environmental signals.
Transcription Can Produce Different Kinds...
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