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Fast Generation of F(Ab')2 Fragments From Human IgG Using Fc-Fused IgG-Degrading Enzyme

Emna Hannachi1, Robin V Lacombe1, Amélia Trecco1

  • 1Institut National de La Santé et De La Recherche Médicale, Centre de Recherche des Cordeliers, CNRS, Sorbonne Université, Université Paris Cité, Paris, France.

European Journal of Immunology
|July 2, 2026
PubMed

Removal of Fc-fragments from human IgG or Fc-fused proteins. (1) IgG or Fc-fusion proteins are incubated with IdeS-Fc; (2) Fc fragments are retained using protein G agarose beads; and (3) the F(ab')2 fragments or Fc-free proteins are collected by centrifugation in the original buffer without IdeS contamination.

Keywords:
F(ab’)2 fragmentsFc‐fused IdeSFc‐fused proteinsIdeSimlifidasepurification

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