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Reprogramming the enduracidin NRPS assembly line via thioesterase domain relocation

Yanan Sun1, Guibin Tu1, Xiaocan Sun1

  • 1Key Laboratory of Industrial Fermentation Microbiology, College of Biotechnology, Tianjin University of Science & Technology, Tianjin, 300457, PR China.

Synthetic and Systems Biotechnology
|July 2, 2026
PubMed
Summary

Repositioning a thioesterase (TE) domain in large nonribosomal peptide synthetase (NRPS) assembly lines enables the creation of novel macrocyclic peptides. This engineering strategy allows for programmed backbone lengths and tailored ring sizes in cyclic peptide libraries.

Keywords:
EnduracidinMacrocyclic peptidesNonribosomal peptide synthetase (NRPS)Thioesterase (TE) domain

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