Development and characterization of a monoclonal antibody against Pseudorabies virus glycoprotein B and its
Hai-Ming Wang1, Yue Sun2, Yu Wang2
1Jiangsu Agri-Animal Husbandry Vocational College, Jiangsu, China.
Abstract:
Pseudorabies virus (PRV) is a swine herpesvirus that causes severe economic losses in the global pig industry. Glycoprotein B (gB) is a highly conserved envelope glycoprotein of PRV and plays an essential role in viral entry, cell fusion, and antibody induction. Here, the extracellular domain of gB from PRV HeN1 strain was expressed in CHO cells and purified. After mouse immunization and hybridoma fusion, a gB-specific monoclonal antibody 1G4 was identified via ELISA and IFA. 1G4 presented high specificity, good reactivity and broad cross-recognition against multiple PRV strains. FITC-labeled 1G4, combined with a gD-targeted mAb, was applied to visualize PRV adsorption and internalization in HeLa cells by confocal microscopy at fixed time points. In conclusion, this novel gB mAb serves as a reliable reagent for PRV mechanism research and immunological detection.
