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CRISPR

CRISPR

Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced Short...

CRISPR

CRISPR

Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced Short...

CRISPR and crRNAs

CRISPR and crRNAs

Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...

CRISPR/Cas9 Genome Editing

CRISPR/Cas9 Genome Editing

The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...

The Antiviral System of Bacteria and Archaea: CRISPR

The Antiviral System of Bacteria and Archaea: CRISPR

CRISPR stands for Clustered Regularly Interspaced Short Palindromic Repeats is a adaptive immune system found in bacteria and archaea that protects against viral infections. This system enables prokaryotic cells to identify, remember, and neutralize foreign genetic elements, primarily bacteriophages, by storing fragments of the invader’s DNA as a genetic memory.The CRISPR immune response begins during an initial infection. Cas (CRISPR-associated) proteins play a central role in this defense.

Modern Molecular Taxonomy

Modern Molecular Taxonomy

Advancements in molecular biology have revolutionized the identification and characterization of bacteria, with multiple methods leveraging DNA sequencing for enhanced precision. As sequencing technologies improve and costs decline, these approaches are increasingly used in clinical, environmental, and evolutionary studies.Multilocus Sequence Typing (MLST) examines several housekeeping genes, essential chromosomal genes encoding cellular functions, to distinguish strains. Approximately...

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Related Experiment Video

Updated: Jul 3, 2026

Point-of-care CRISPR-based Diagnostics with Premixed and Freeze-dried Reagents

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Published on: August 16, 2024

CRISPR-based next-generation molecular diagnostics for bone infection.

Ju Chen¹, Hao Tan¹, Yu Guo¹

¹Clinical Medical College and Affiliated Hospital, Chengdu University, Chengdu, China.

Frontiers in Cell and Developmental Biology

|July 2, 2026

Summary

This summary is machine-generated.

Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) diagnostics offer sensitive and specific detection for bone infections. This technology promises to overcome limitations of traditional methods, enabling faster, targeted antimicrobial therapy and improved patient outcomes.

Keywords:

AMR (antimicrobial resistance)CRISPR-diagnosics POCT (point-of-care testing)bone infection osteomyelitis (OM)

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Rapid and Specific Detection of Acinetobacter baumannii Infections Using a Recombinase Polymerase Amplification/Cas12a-based System

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Area of Science:

Orthopedic diagnostics
Molecular biology
Infectious disease detection

Background:

Bone and joint infections like osteomyelitis pose significant clinical challenges due to diagnostic uncertainty and rising antimicrobial resistance (AMR).
Conventional culture methods often yield false-negative results (up to 50%), hindering effective management.
Existing molecular diagnostics struggle with sensitivity and specificity for complex orthopedic infections.

Purpose of the Study:

To review the current diagnostic landscape for bone infections, emphasizing limitations of conventional and early molecular techniques.
To explore the potential of Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)-associated (Cas) technologies for diagnosing orthopedic infections.
To identify research priorities for translating CRISPR diagnostics into routine orthopedic practice.

Main Methods:

Critical evaluation of existing diagnostic methods for bone infections.
Examination of CRISPR-Cas systems (Cas12, Cas13, Cas14, CasΦ) for nucleic acid detection.
Analysis of CRISPR applications in pathogen stratification, AMR gene identification, and point-of-care testing.

Main Results:

CRISPR-Cas systems offer attomolar sensitivity and single-nucleotide specificity, compatible with portable formats.
Cas effectors enable SNP discrimination, RNA-based viability assessment, and PAM-independent detection.
CRISPR technology can stratify pathogens, identify AMR genes, and facilitate intraoperative point-of-care testing.

Conclusions:

CRISPR diagnostics represent a promising alternative to conventional methods for bone and joint infections.
Addressing challenges in sample processing, standardization, and clinical validation is crucial for widespread adoption.
Further research is needed to transition CRISPR technology from proof-of-concept to routine clinical use in orthopedics.