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Related Experiment Video

Updated: Jul 3, 2026

Digestion of Whole Mouse Eyes for Multi-Parameter Flow Cytometric Analysis of Mononuclear Phagocytes
09:58

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Published on: June 17, 2020

Infiltrating monocytes augment alternative complement activation and exacerbate inherited retinal degeneration in a

Wenxin Ma1, Quyan Zhang1, Pinghu Liu2

  • 1Retinal Neurophysiology Section.

Research Square
|July 2, 2026
PubMed
Summary

Infiltrating monocytes worsen retinal degeneration by promoting inflammation. Targeting these cells reduced inflammation and protected photoreceptors in a mouse model, offering a potential neuroprotective therapy.

Keywords:
C3CCR2-CreERCFHDTAMicrogliaMüller cellmacrophagemonocyterd10

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Related Experiment Videos

Last Updated: Jul 3, 2026

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09:58

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Published on: June 17, 2020

Efficient Dissection and Culture of Primary Mouse Retinal Pigment Epithelial Cells
08:33

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Published on: February 10, 2021

In Vivo Imaging of Cx3cr1gfp/gfp Reporter Mice with Spectral-domain Optical Coherence Tomography and Scanning Laser Ophthalmoscopy
06:19

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Published on: November 11, 2017

Area of Science:

  • Neuroscience
  • Immunology
  • Ophthalmology

Background:

  • Microglia and macrophages accumulate in retinal degenerative diseases, influencing progression.
  • Distinct roles of these immune cells in retinal degeneration are not fully understood.

Purpose of the Study:

  • To define the fate and function of infiltrating monocyte-derived macrophages (MDM) in retinal degeneration.
  • To investigate the impact of these cells on disease progression and explore therapeutic potential.

Main Methods:

  • Generated a CCR2-CreER mouse line on the rd10 background for monocyte tracking and ablation.
  • Utilized immunohistochemistry and transcriptomic profiling to analyze cell behavior and gene expression.
  • Performed selective ablation of infiltrating monocytes to assess functional outcomes.

Main Results:

  • Infiltrating monocytes acquired microglia-like characteristics (TMEM119+, P2RY12+) and were partially cleared by resident microglia.
  • Microglia-monocyte interactions promoted Müller cell C3 production and alternative complement pathway activation.
  • Selective monocyte ablation reduced microglial activation, inflammation, complement deposition, and ameliorated photoreceptor degeneration.

Conclusions:

  • Infiltrating monocytes are key drivers of immune dysregulation and inflammation in retinal degeneration.
  • Targeting infiltrating monocytes shows therapeutic potential for neuroprotection in retinal degenerative diseases.