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Related Concept Videos

Introduction to Mechanisms of Enzyme Catalysis01:13

Introduction to Mechanisms of Enzyme Catalysis

For many years, scientists thought that enzyme-substrate binding took place in a simple "lock-and-key" fashion. This model stated that the enzyme and substrate fit together perfectly in one instantaneous step. However, current research supports a more refined view scientists call induced fit. The induced-fit model expands upon the lock-and-key model by describing a more dynamic interaction between enzyme and substrate. As the enzyme and substrate come together, their interaction causes a mild...
Introduction to Mechanisms of Enzyme Catalysis01:13

Introduction to Mechanisms of Enzyme Catalysis

For many years, scientists thought that enzyme-substrate binding took place in a simple "lock-and-key" fashion. This model stated that the enzyme and substrate fit together perfectly in one instantaneous step. However, current research supports a more refined view scientists call induced fit. The induced-fit model expands upon the lock-and-key model by describing a more dynamic interaction between enzyme and substrate. As the enzyme and substrate come together, their interaction causes a mild...
Microbial Corrosion01:24

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Microbiologically Influenced Corrosion (MIC) is a significant form of material degradation caused by the metabolic activities of microorganisms. This phenomenon poses substantial challenges across various industries, including oil and gas, maritime, and water treatment sectors.MIC occurs when microorganisms, such as bacteria, archaea, and fungi, colonize metal surfaces, forming biofilms that alter the local electrochemical environment. These biofilms can lead to the production of corrosive...
Catalytically Perfect Enzymes01:07

Catalytically Perfect Enzymes

The theory of catalytically perfect enzymes was first proposed by W.J. Albery and J. R. Knowles in 1976. These enzymes catalyze biochemical reactions at high-speed. Their catalytic efficiency values range from 108-109 M-1s-1. These enzymes are also called 'diffusion-controlled' as the only rate-limiting step in the catalysis is that of the substrate diffusion into the active site. Examples include triose phosphate isomerase, fumarase, and superoxide dismutase.
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Microbial Bioremediation of Plastics

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Updated: Jul 3, 2026

Multi-enzyme Screening Using a High-throughput Genetic Enzyme Screening System
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Deploying Artificial Metalloenzymes in Complex Environments: Strategies and Applications.

Yuan Yao1, Wei Su1, Tong Wu1

  • 1State Key Laboratory of Chemo- and Bio-Sensing, School of Chemistry and Chemical Engineering, Hunan University, Changsha, China.

Chembiochem : a European Journal of Chemical Biology
|July 2, 2026
PubMed
Summary

Artificial metalloenzymes (ArMs) are engineered catalysts with broad applications. This review explores strategies to enhance ArM stability and function in complex biological settings for diverse research and therapeutic uses.

Keywords:
abiotic transformationartificial metalloenzymebioorthogonal chemistrycell lysateswhole‐cell catalysis

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Published on: August 23, 2018

Area of Science:

  • Biochemistry
  • Chemical Biology
  • Synthetic Biology

Background:

  • Artificial metalloenzymes (ArMs) are engineered proteins with novel catalytic functions.
  • ArMs offer potential in synthetic biology, chemical biology, and biomedical research.
  • Robustness is crucial for ArM application in complex biological environments.

Purpose of the Study:

  • To review strategies for stabilizing ArMs.
  • To discuss ArM adaptation for cell lysates, whole-cell catalysts, and in vivo applications.
  • To highlight design principles, breakthroughs, and future opportunities for robust ArMs.

Main Methods:

  • Review of established strategies for ArM stabilization.
  • Analysis of ArM integration into cell lysates.
  • Examination of ArM application in whole-cell catalysis and in vivo studies.

Main Results:

  • Strategies exist to stabilize ArMs for various biological environments.
  • ArMs can be adapted for use in cell lysates, whole cells, and live animals.
  • Successful stabilization enhances ArM performance in complex settings.

Conclusions:

  • Robust ArMs are essential for advancing synthetic biology, chemical biology, and therapeutics.
  • Further research into design principles and stabilization techniques will expand ArM utility.
  • ArMs show promise as versatile tools in biological and medical research.