Related Experiment Video
Updated: Jul 3, 2026
Preparation of a Blood Culture Pellet for Rapid Bacterial Identification and Antibiotic Susceptibility Testing
Published on: October 15, 2014
Prevalence and Antimicrobial Resistance of Non fermenting Gram-negative Bacilli Isolated from Blood Culture Samples
Ankita Poddar1, Yugeshwari R Tiwade2, Shibanand Nepal Karmakar3
1Department of Microbiology, Datta Meghe Medical College, Datta Meghe Institute of Higher Education and Research (DU), Wardha, Maharashtra, India.
Background:
Non-fermenting Gram-negative bacilli (NFGNB) are emerging as a significant cause of bloodstream infections (BSIs), a major concern of morbidity and mortality worldwide. The high intrinsic resistance of NFGNB to antimicrobial compounds makes treatment of BSI difficult and expensive.
Aims:
This study aimed to determine the prevalence of NFGNB and the antimicrobial susceptibility pattern of the isolates.
Methodology:
A total of 1230 blood culture samples were received in the department of microbiology during the study period of 3 months, which were processed by standard microbiological procedures. Antimicrobial susceptibility tests were performed using Clinical and Laboratory Standards Institute (CLSI) guidelines.
Results:
Out of 255 (20.73%) positive blood culture samples, 105 (8.5%) Gram-negative bacilli were isolated, of which 41 (3.33%) NFGNB were identified. The most common isolate identified is Pseudomonas aeruginosa (21), followed by Acinetobacter spp. (20). A higher percentage of antibiotic resistance was seen for Acinetobacte r spp. and even higher antibiotics like ceftazidime (87.5%) and gentamicin (92.5%).
Conclusion:
This study will help us start empirical treatment and implement proper hospital infection control measures.
More Related Videos
08:30One-day Workflow Scheme for Bacterial Pathogen Detection and Antimicrobial Resistance Testing from Blood Cultures
Published on: July 9, 2012
08:58Isolation and Identification of Waterborne Antibiotic-Resistant Bacteria and Molecular Characterization of their Antibiotic Resistance Genes
Published on: March 3, 2023
Related Concept Videos
01:25Clinical Significance of Antibiotic Resistance
01:30Development of Antibiotic Resistance
01:25Mechanism of Antibiotic Resistance in MRSA
01:26Urine Studies II: Urine Culture and Sensitivity Test
01:28Antimicrobial Effectiveness
01:20Sputum Studies II: Culture and Sensitivity
Sputum culture and sensitivity is a medical procedure used to diagnose bacterial infections in the respiratory tract and select the most appropriate antibiotics for treatment. This process involves analyzing sputum samples of thick and opaque secretions produced in the lungs and airways. These samples are collected from patients and then sent to the laboratory for analysis.
The test can identify various pathogens responsible for respiratory infections, including Streptococcus,...