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Imaging Biological Samples with Optical Microscopy

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Related Experiment Video

Updated: Jul 3, 2026

Multimodal Optical Imaging Platform for Studying Cellular Metabolism
04:47

Multimodal Optical Imaging Platform for Studying Cellular Metabolism

Published on: June 6, 2025

Single-cell mass spectrometry imaging: platform advances for multimodal spatial omics.

Lauren E Hill1, Lyndsay E A Young1, James W Dressman1

  • 1Department of Pharmacology and Immunology, Medical University of South Carolina, Charleston, SC, 29425, USA.

Analytical and Bioanalytical Chemistry
|July 2, 2026
PubMed
Summary

Single-cell mass spectrometry imaging (MSI) offers label-free, in situ molecular visualization for understanding cellular heterogeneity. This review highlights advancements in single-cell MSI platforms and their potential for translational research and precision medicine.

Keywords:
MALDI-MSIMass spectrometry imagingSingle-cell imagingSpatial omics

Related Experiment Videos

Last Updated: Jul 3, 2026

Multimodal Optical Imaging Platform for Studying Cellular Metabolism
04:47

Multimodal Optical Imaging Platform for Studying Cellular Metabolism

Published on: June 6, 2025

Area of Science:

  • Biomedical Research
  • Molecular Biology
  • Analytical Chemistry

Background:

  • Spatial omics preserves spatial context for molecular characterization.
  • Mass spectrometry imaging (MSI) enables label-free, in situ visualization of diverse molecules.
  • Advances in MSI are enabling single-cell analysis, revealing cellular heterogeneity.

Purpose of the Study:

  • To review current single-cell MSI platforms.
  • To highlight innovations improving spatial resolution, sensitivity, and throughput.
  • To discuss the potential of single-cell MSI in translational research and precision medicine.

Main Methods:

  • Review of current single-cell mass spectrometry imaging (MSI) platforms.
  • Highlighting key innovations in instrumentation, sample preparation, and data acquisition.
  • Focus on desorption electrospray ionization (DESI), secondary ion mass spectrometry (SIMS), and matrix-assisted laser desorption ionization (MALDI) techniques.

Main Results:

  • Single-cell MSI provides unprecedented access to cellular heterogeneity and molecular states.
  • Variability exists in cell isolation, capture, and data acquisition strategies.
  • DESI, SIMS, and MALDI enable robust single-cell multi-omics profiling.

Conclusions:

  • Single-cell MSI is a powerful tool for understanding biological systems at the cellular level.
  • Future directions aim to further enhance capabilities for translational applications.
  • Single-cell MSI holds significant potential for advancing precision medicine.