CUT&Tag Epigenomic Profiling of Biosynthetic Gene Clusters in Arabidopsis thaliana

Plants synthesise a diverse array of specialised metabolites with important functions in environmental adaptation and reproduction. Biosynthesis genes arranged in genomic clusters typically exhibit shared epigenomic features that likely contribute to their transcriptional co-regulation. Here, we describe a protocol for the genome-wide profiling of histone modifications in Arabidopsis thaliana by CUT&Tag. In addition, we provide an overview about the bioinformatic analysis of tissue-specific enrichment patterns of epigenetic marks within biosynthetic gene clusters.