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Updated: Jul 4, 2026
Purification of High Yield Extracellular Vesicle Preparations Away from Virus
Published on: September 12, 2019
Protocol for isolating human extracellular vesicles from nephrotic urine and enriching autoimmunoglobulin-triggered
Karen Lahme1, Catherine Meyer-Schwesinger1
1Institute of Cellular and Integrative Physiology, Center for Experimental Medicine, University Medical Center Hamburg-Eppendorf (UKE), 20246 Hamburg, Germany; Hamburg Center of Kidney Health, University Medical Center Hamburg-Eppendorf (UKE), 20246 Hamburg, Germany.
Abstract:
This protocol describes the isolation of total urinary extracellular vesicles (EVs) from nephrotic patient urines and enrichment of AutoImmunoglobulin-Triggered Extracellular Vesicles (AIT-EVs). Procedures for urine collection, sample processing, buffer preparation, antibody coupling to DynaBeads, urine ultrafiltration, and total EV isolation are provided. The protocol further details EV concentration measurement and selective AIT-EV capture and elution using anti-human IgG4 antibodies. This approach enables reproducible enrichment of AIT-EVs and can be adapted to isolate other EV subtypes by substituting the capture antibody.
