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Updated: Jul 4, 2026
Mechanical Separation and Protein Solubilization of the Outer and Inner Perivitelline Sublayers from Hen's Eggs
Published on: January 27, 2021
Protocol for in vitro phase separation of ARID3B proteins
Wenyu Han1, Xiaofeng Li1, Junyan Lin1
1Department of Orthodontics, The Affiliated Stomatological Hospital of Nanjing Medical University, Nanjing, China; State Key Laboratory Cultivation Base of Research, Prevention and Treatment for Oral Diseases (Nanjing Medical University), Nanjing, China; Jiangsu Province Engineering Research Center of Stomatological Translational Medicine (Nanjing Medical University), Nanjing, China.
Abstract:
Liquid-liquid phase separation (LLPS) of ARID3B is critical in nonsyndromic cleft lip/palate pathogenesis. Here, we present a protocol to visualize ARID3B LLPS. We describe steps for regenerating LLPS with purified protein in vitro and detecting ARID3B condensates in mammalian cells. This protocol enables analysis of ARID3B phase separation dynamics and the study of other transcription factors that undergo LLPS. For complete details on the use and execution of this protocol, please refer to Li et al.1.
