Protocol for inducing deep vein thrombosis in C57BL/6J mice using the inferior vena cava stenosis model

Here, we present a protocol for inducing deep vein thrombosis (DVT) in C57BL/6J mice using the inferior vena cava (IVC) stenosis model. We describe steps for surgical preparation, IVC ligation, and non-invasive detection and confirmation of thrombus development using high-frequency ultrasound (HFUS). We then detail procedures to dissect thrombi, prepare single-cell suspensions, and analyze infiltrating myeloid cells, including monocytes, by flow cytometry. This protocol allows precise timing, visualization, and immune profiling of early thrombus formation within 48 h. For complete details and execution of this protocol, please refer to Shahneh et al.^1,2.