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Related Concept Videos

Single-Strand DNA Binding Proteins01:03

Single-Strand DNA Binding Proteins

For successful DNA replication, the unwinding of double-stranded DNA must be accompanied by stabilization and protection of the separated single strands of the DNA. This crucial task is performed by single-strand DNA-binding (SSB) proteins. They bind to the DNA in a sequence-independent manner, which means that the nitrogenous bases of the DNA need not be present in a specific order for binding of SSB proteins to it. The binding of SSB proteins straightens single-stranded DNA (ssDNA) and makes...
The Replisome03:01

The Replisome

DNA replication is carried out by a large complex of proteins that act in a coordinated matter to achieve high-fidelity DNA replication. Together this complex is known as the DNA replication machinery or the replisome.
The synthesis of the leading and lagging strands is a highly coordinated process. To explain this, the “Trombone model” was proposed by Bruce Alberts in 1980. The DNA loop formation starts when a primer is synthesized on the parent lagging strand. The loop grows with the...
The Replisome03:01

The Replisome

DNA replication is carried out by a large complex of proteins that act in a coordinated matter to achieve high-fidelity DNA replication. Together this complex is known as the DNA replication machinery or the replisome.
The synthesis of the leading and lagging strands is a highly coordinated process. To explain this, the “Trombone model” was proposed by Bruce Alberts in 1980. The DNA loop formation starts when a primer is synthesized on the parent lagging strand. The loop grows with the...
The DNA Replication Fork01:02

The DNA Replication Fork

An organism’s genome needs to be duplicated in an efficient and error-free manner for its growth and survival. The replication fork is a Y-shaped active region where two strands of DNA are separated and replicated continuously. The coupling of DNA unzipping and complementary strand synthesis is a characteristic feature of a replication fork.   Organisms with small circular DNA, such as E. coli, often have a single origin of replication; therefore, they have only two replication forks, one in...
The DNA Replication Fork01:02

The DNA Replication Fork

An organism’s genome needs to be duplicated in an efficient and error-free manner for its growth and survival. The replication fork is a Y-shaped active region where two strands of DNA are separated and replicated continuously. The coupling of DNA unzipping and complementary strand synthesis is a characteristic feature of a replication fork.   Organisms with small circular DNA, such as E. coli, often have a single origin of replication; therefore, they have only two replication forks, one in...
Replication in Prokaryotes01:32

Replication in Prokaryotes

DNA replication has three main steps: initiation, elongation, and termination. Replication in prokaryotes begins when initiator proteins bind to the single origin of replication (ori) on the cell's circular chromosome. Replication then proceeds around the entire circle of the chromosome in each direction from the two replication forks, resulting in two DNA molecules.
Many Proteins Work Together to Replicate the Chromosome
Replication is coordinated and carried out by a host of specialized...

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Related Experiment Video

Updated: Jul 4, 2026

Strand-Specific Analysis of Proteins at Replicating DNA Strands by Enrichment and Sequencing of Protein-Associated Nascent DNA Method
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Strand-Specific Analysis of Proteins at Replicating DNA Strands by Enrichment and Sequencing of Protein-Associated Nascent DNA Method

Published on: May 2, 2025

Bacillus subtilis DnaB forms multiple protein-protein interactions essential for DNA replication initiation.

Aurélie Guyet1, Reyes Ruiz Campoy2, Petra Manja3

  • 1Centre for Bacterial Cell Biology, Biosciences Institute, Newcastle University, Newcastle Upon Tyne NE2 4AX, United Kingdom.

Nucleic Acids Research
|July 2, 2026
PubMed
Summary
This summary is machine-generated.

This study reveals how the DnaB protein orchestrates DNA replication initiation in Bacillus subtilis by interacting with key proteins DnaA, DnaD, and DnaI, ensuring dual helicase loading at the origin.

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Last Updated: Jul 4, 2026

Strand-Specific Analysis of Proteins at Replicating DNA Strands by Enrichment and Sequencing of Protein-Associated Nascent DNA Method
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Determination of the Optimal Chromosomal Location(s) for a DNA Element in Escherichia coli Using a Novel Transposon-mediated Approach
11:12

Determination of the Optimal Chromosomal Location(s) for a DNA Element in Escherichia coli Using a Novel Transposon-mediated Approach

Published on: September 11, 2017

Area of Science:

  • Molecular Biology
  • Microbiology
  • Biochemistry

Background:

  • DNA replication initiation occurs at specific origins (oriC).
  • Bacterial replication involves the master initiator DnaA and replicative helicases.
  • Mechanisms of bacterial helicase loading at oriC remain unclear.

Purpose of the Study:

  • Investigate the role of the essential accessory helicase loader DnaB in Bacillus subtilis.
  • Identify critical DnaB residues and protein-protein interactions in DNA replication initiation.

Main Methods:

  • Characterization of DnaB residues.
  • Single particle cryo-electron microscopy.
  • AlphaFold3 predictions and two-hybrid interaction analyses.

Main Results:

  • Identified three essential protein-protein interactions for DnaB with DnaA, DnaD, and DnaI.
  • DnaB functions as an interaction hub.
  • Data suggests DnaB orchestrates dual helicase loading at the origin.

Conclusions:

  • Proposed a model for DNA replication initiation in B. subtilis and related Firmicutes pathogens.
  • DnaB acts as a crucial orchestrator for helicase loading during replication initiation.