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Advances and perspectives in Oligo(dT) Affinity chromatography for mRNA capture: Resins, ligands and process
Xuena Tian1, Jiayang Jin1, Xuan Zhu1
1State Key Laboratory of Bioreactor Engineering, School of Biotechnology, East China University of Science and Technology, Shanghai 200237, China; Shanghai Collaborative Innovation Center for Biomanufacturing Technology (SCICBT), Shanghai 200237, China.
Abstract:
With the rapid development of the mRNA pharmaceutical industry, Oligo(dT) chromatography, as a core step in mRNA separation and purification, has garnered increasing attention from both the industry and researchers. This article begins with the fundamental principles of mRNA affinity chromatography, analyzes the advantages and disadvantages of two typical affinity techniques, and highlights the sufficiency of Oligo(dT) chromatography as a platform technology. Aiming to the poor performance of the Oligo(dT) chromatography, the article reviews the main methods and mechanisms for enhancing its performance from three perspectives, resin structure, ligand structure, and process intensification. It also highlights the factors influencing the performance of Oligo(dT) chromatography and the challenges associated with these methods. The article provides an outlook on the future development directions of Oligo(dT) chromatography, offering insights for the advancement of next-generation Oligo(dT) chromatography resins and processes.
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