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Related Concept Videos

Microbiota Modulation by Antibiotics01:21

Microbiota Modulation by Antibiotics

Antibiotics have revolutionized modern medicine by saving countless lives from bacterial infections. However, their widespread use has inadvertently harmed the delicate balance of the human gut microbiota. The gut microbiota, a complex community of bacteria, archaea, viruses, and fungi, plays a vital role in regulating metabolism, immune responses, and maintaining intestinal health. Antibiotics, especially broad-spectrum types, disrupt this ecosystem by eradicating both harmful and beneficial...
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Dysbiosis of the Gut Microbiota

The human gut microbiome includes a diverse array of microbial species, including beneficial commensals and opportunistic pathogens, which interact to support host health. These microbes contribute to essential functions such as nutrient metabolism, immune system modulation, and maintenance of intestinal barrier integrity. However, disruptions to this equilibrium—referred to as dysbiosis—can have widespread physiological consequences.Dysbiosis is often characterized by reduced microbial...
Development of Human Microbiota01:30

Development of Human Microbiota

The human microbiota begins developing at birth and undergoes continual change as we age. Infancy marks a critical period of microbial sensitivity, offering a “window of opportunity” during which beneficial microbes help mature the immune system. By age three, children typically develop a more stable and diverse microbial community. Newborns acquire microbes from their immediate environment; vaginal delivery favors maternal vaginal microbes, while cesarean births favor microbes from the skin...

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Related Experiment Video

Updated: Jul 4, 2026

Quantitative Polymerase Chain Reaction-based Analyses of Murine Intestinal Microbiota After Oral Antibiotic Treatment
08:33

Quantitative Polymerase Chain Reaction-based Analyses of Murine Intestinal Microbiota After Oral Antibiotic Treatment

Published on: November 17, 2018

Distinct compositional changes but shared quantitative microbiome and anti-inflammatory modulations by diet.

Hannah K Ruple1, Lynn Schintgen1, Eva Haasis2

  • 1Department of Microbiome Research and Applied Bioinformatics, Institute of Nutritional Sciences, University of Hohenheim, Stuttgart, Germany.

Cell Reports
|July 2, 2026
PubMed
Summary

Quantitative microbiome parameters (QMPs) are influenced by diet and feeding patterns. Specific interventions like high-fiber diets and time-restricted feeding reduce gut transit time and microbial load, suggesting diagnostic and therapeutic potential for microbiome-mediated diseases.

Keywords:
CP: metabolismCP: microbiologyIL-10 deficiencyfastinghigh-fat diethigh-fiber dietintestinal inflammationquantitative microbiome analysistaxonomic microbiota analysistime-restricted feeding

Related Experiment Videos

Last Updated: Jul 4, 2026

Quantitative Polymerase Chain Reaction-based Analyses of Murine Intestinal Microbiota After Oral Antibiotic Treatment
08:33

Quantitative Polymerase Chain Reaction-based Analyses of Murine Intestinal Microbiota After Oral Antibiotic Treatment

Published on: November 17, 2018

Area of Science:

  • Microbiome research
  • Gastrointestinal physiology
  • Immunology

Background:

  • Gut microbiome composition is inconsistently linked to human diseases.
  • Understanding factors controlling microbiome parameters is crucial for disease association studies.

Purpose of the Study:

  • To investigate the influence of diet and feeding patterns on quantitative microbiome parameters (QMPs).
  • To explore the role of IL-10 deficiency in modulating QMPs.
  • To assess the relationship between QMPs, gene expression, and gastrointestinal transit time.

Main Methods:

  • Utilized a food dye-based gastrointestinal (GI) passage assay in mice.
  • Measured GI transit time, fecal mass, microbial load, and microbiota proliferation.
  • Analyzed intestinal gene expression signatures under different dietary and feeding conditions.

Main Results:

  • Diet, feeding pattern, and IL-10 deficiency significantly controlled QMPs.
  • High-fiber diet (HFiD) and time-restricted feeding (TRF) reduced GI transit time and QMPs.
  • High-fat diet (HFaD) showed contrary effects on QMPs.
  • Intestinal gene expression supported anti-inflammatory effects of HFiD and TRF, contrasting with taxonomic profiles.

Conclusions:

  • QMPs are distinct from taxonomic profiles and are modulated by diet and feeding patterns.
  • HFiD and TRF induce convergent QMP and gene expression changes, suggesting shared mechanisms.
  • QMPs show potential for diagnosing and treating microbiome-mediated diseases.