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Related Experiment Video

Updated: Jul 4, 2026

Tissue-specific miRNA Expression Profiling in Mouse Heart Sections Using In Situ Hybridization
08:22

Tissue-specific miRNA Expression Profiling in Mouse Heart Sections Using In Situ Hybridization

Published on: September 15, 2018

Differential cardiac microRNA expression in anoxic Trachemys scripta elegans turtles.

Tighe Bloskie1, Olawale O Taiwo1, William G Willmore1

  • 1Institute of Biochemistry and Department of Biology, Carleton University, 1125 Colonel By Drive, Ottawa, Ontario, K1S 5B6, Canada.

Biochimie
|July 2, 2026
PubMed
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Red-eared slider turtles exhibit remarkable anoxia tolerance. MicroRNAs (miRNAs) play a key role in cardiac adaptation by regulating gene expression during oxygen deprivation, aiding metabolic suppression and cellular resilience.

Area of Science:

  • Cardiovascular Physiology
  • Molecular Biology
  • Genomics

Background:

  • Red-eared slider turtles (Trachemys scripta elegans) display extraordinary tolerance to anoxia.
  • This resilience involves metabolic suppression and transcriptional changes, but the role of microRNAs (miRNAs) is unclear.
  • Understanding miRNA involvement can reveal mechanisms of anoxia tolerance and cardiac protection.

Purpose of the Study:

  • To investigate the role of cardiac miRNAs in the anoxic adaptation of red-eared slider turtles.
  • To identify specific miRNAs that are differentially expressed under anoxic conditions.
  • To explore the potential functions of these miRNAs in cellular responses to oxygen deprivation.

Main Methods:

  • Small RNA sequencing was used to profile cardiac miRNA expression in turtles under normoxic and anoxic conditions (20 hours at 5 °C).
Keywords:
Anoxia toleranceCardiac hypometabolismPost-transcriptional regulationTrachemys scripta elegansmicroRNA

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  • Differential expression analysis was performed on both human-conserved and non-human turtle miRNAs.
  • Gene set enrichment analysis was used to predict the functional roles of identified miRNAs.

    • Main Results:

    • Two human-conserved miRNAs, miR-1973 and miR-6747-5p, showed increased expression under anoxia.
    • Thirty-five non-human miRNAs from Chrysemys picta were identified, with four (cpi-miR-2188-5p, cpi-miR-15b-5p, cpi-miR-551-5p, cpi-miR-1805-3p) showing significant anoxia-responsive changes.
    • Predicted functions include protein modification, cytoskeletal remodeling, and metabolic adaptation via ribosome suppression.

    Conclusions:

    • miRNAs contribute to cardiac plasticity and anoxia tolerance in T.s. elegans.
    • These miRNAs likely modify cellular phenotype and reduce energy expenditure during oxygen deprivation.
    • The findings offer insights into post-transcriptional regulation of vertebrate anoxia tolerance and potential applications in ischemia-reperfusion medicine.