[A new phorbol compound from bidirectional fermentation product "Lingba Junzhi" of Ganoderma lucidum and Crotonis...

Ten compounds were isolated from the bidirectional fermentation product "Lingba Junzhi"(LBJZ) of Ganoderma lucidum and Crotonis Fructus through a combination of chromatographic techniques, including silica gel column chromatography, thin-layer chromatography, and high-performance liquid chromatography. Their chemical structures were elucidated by spectroscopic analyses, including high-resolution mass spectrometry and one-dimensional/two-dimensional nuclear magnetic resonance spectroscopy. These compounds were identified as 12-O-pentenoyl-13-O-acetylphorbol(1), 12-O-tigloylphorbol-13-isobutyrate(2), phorbol-13-dodecanoate(3), 12-O-tigloylphorbol-13-acetate(4), phorbol-13-acetate(5), 4-deoxy-4α-phorbol-13-acetate(6), adenosine(7), arabitol(8), crotonoside(9), and uridine(10). Among them, compound 1 was a new compound, while compounds 2-10 were reported for the first time from LBJZ.