[A new terpenoid from Paeoniae Radix Rubra and its anticoagulant activity in vitro]

Twenty-one compounds were isolated and identified from the 80% ethanol extract of Paeoniae Radix Rubra by various chromatographic techniques such as D101 macroporous resin, C_(18) reversed-phase silica gel, and gel chromatography, combined with modern spectroscopic techniques including infrared spectroscopy(IR), ultraviolet(UV), mass spectrometry(MS), and nuclear magnetic resonance(NMR). These compounds were 3-formyl-5-hydroxy-6-methyl-1H-indole-1-butyric acid(1), 3-hydroxymethyl-5-methyl-2,3-dihydrobenzofuran(2), paeoniflorin(3), 5-hydroxy-6-methyl-1H-indole-3-carbaldehyde(4), 5-hydroxy-3S-hydroxymethyl-6-methyl-2,3-dihydrobenzofuran(5), paeoveitol C(6), galloylpaeoniflorin(7), benzoylpaeoniflorin(8), 4-O-galloylpaeoniflorin(9), 1-borneol 6-O-[β-D-apiofuranosyl-(1→6)]-β-D-glucopyranoside(10), paeonidanin(11), paeonidanin E(12), paeonidanin B(13), palbinone(14), 23-hydroxybetulinic acid(15), hederagenin(16), N-methylhydroxylamine(17),(5Z,9Z)-17-methylnonadeca-5,9-dienoate(18), 3,3',4'-tri-O-methylellagic acid(19), β-sitosterylglucoside-6'-octadecanoate(20), and trilinolein(21). Among them, compound 1 was a new compound, and compounds 10 and 17-21 were isolated from the Paeoniae genus for the first time. All the compounds were tested for their anticoagulant activity through three coagulation function indicators: activated partial thromboplastin time(APTT), thrombin time(TT), and prothrombin time(PT). Compounds 1 and 7 showed excellent anticoagulant activity, which was comparable to that of the positive control sodium heparin. Compounds 2, 3, 5, 6, 8-13, 15, and 19 exhibited good anticoagulant activity, while compounds 4 and 21 demonstrated moderate anticoagulant activity.