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Updated: Jul 4, 2026

Anti-Nuclear Antibody Screening Using HEp-2 Cells
Published on: June 23, 2014
[Anti-inflammatory 2-(2-phenylethyl)chromone dimers from Aquilariae Lignum Resinatum]
Hui-Ting Liu1, Jiao-Jiao Yin1, Yue-Yang Cao1
1School of Chinese Materia Medica, Beijing University of Chinese Medicine Beijing 102488, China Modern Research Center for TCM, Beijing Institute of Chinese Medicine, Beijing University of Chinese Medicine Beijing 102488, China.
None:
Four 2-(2-phenylethyl)chromone dimers were isolated from EtOAc extract of Aquilariae Lignum Resinatum by various column chromatography techniques including silica gel, ODS, Sephadex LH-20, and semi-preparative high performance liquid chromatography(HPLC). Their structures were identified as(+)-aquisinenone T(1), wallone D(2), 6″-hydroxy-crassin B(3), and 6″-hydroxy-4-methoxy-crassin B(4) on the basis of physicochemical properties and ultraviolet spectroscopy(UV), infrared spectroscopy(IR), high-resolution electrospray ionization mass spectrometry(HR-ESI-MS), and nuclear magnetic resonance(NMR) spectroscopic data. Among them, compound 1 was characterized as a new 2-(2-phenylethyl)chromone dimer. The isolated compounds were screened for their anti-inflammatory activities. Compounds 1, 3, and 4 significantly inhibited nitric oxide production in lipopolysaccharide-stimulated RAW264.7 cells with IC_(50) values of(27.96±6.22),(39.06±1.33), and(37.27±1.21) μmol·L~(-1), respectively.
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