Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Responses to Salt Stress02:02

Responses to Salt Stress

Salt stress—which can be triggered by high salt concentrations in a plant’s environment—can significantly affect plant growth and crop production by influencing photosynthesis and the absorption of water and nutrients.
Key Elements for Plant Nutrition02:35

Key Elements for Plant Nutrition

Like all living organisms, plants require organic and inorganic nutrients to survive, reproduce, grow and maintain homeostasis. To identify nutrients that are essential for plant functioning, researchers have leveraged a technique called hydroponics. In hydroponic culture systems, plants are grown—without soil—in water-based solutions containing nutrients. At least 17 nutrients have been identified as essential elements required by plants. Plants acquire these elements from the atmosphere, the...
Overview of Metabolism01:40

Overview of Metabolism

Living cells constantly carry out various chemical reactions which are necessary for their proper functioning. These reactions are interlinked to one another via multiple pathways. The collection of these chemical reactions is known as metabolism.
Plant Metabolism
Sunlight, the primary source of energy in plants, is first absorbed by the chlorophyll pigments present in their leaves. Plants then use this energy to carry out photosynthesis, where water is oxidized into oxygen and carbon dioxide...
Tonicity in Plants01:20

Tonicity in Plants

Plant cells maintain appropriate osmotic balance in extreme conditions. For instance, plants in dry environments store water in vacuoles, limit the opening of their stoma, and have thick, waxy cuticles to prevent unnecessary water loss. Some species of plants that live in salty environments store salt in their roots. As a result, water osmosis occurs in the root from the surrounding soil.
Tonicity
Tonicity describes the capacity of a cell to lose or gain water depending on the solute...
Global Regulatory Systems01:28

Global Regulatory Systems

Global regulatory systems in bacteria enable rapid and coordinated responses to environmental changes by integrating sensory inputs with gene expression, ensuring efficient adaptation to fluctuating conditions. Key global regulatory mechanisms include regulons, two-component systems, sigma factors, and secondary messengers.Regulons and Global RegulatorsA regulon is a collection of genes and operons controlled by a common global regulator. These regulators enable bacteria to prioritize resource...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A Simplified Wheat Protoplast Transformation System and Guideline for Avoiding Protein Localization Artifacts.

Plants (Basel, Switzerland)·2026
Same author

Identification of <i>SmNAC28</i> Transcription Factor and Its Mechanism of Regulating Salt Tolerance in Eggplant via S-Palmitoylation.

Current issues in molecular biology·2026
Same author

ERF026 balances the growth and drought stress response of Medicago sativa L. by regulating jasmonic acid metabolism.

Plant physiology·2025
Same author

Heterotrimeric G-protein subunits regulate plant architecture, pod development, seed size, and symbiotic nodulation in <i>Medicago truncatula</i>.

aBIOTECH·2025
Same author

Regulation of the immunity-related VIK-APK-EDS1 pathway in Medicago for resistance to Phytophthora.

The Plant cell·2025
Same author

An Aegilops longissima NLR protein with integrated CC-BED module mediates resistance to wheat powdery mildew.

Nature communications·2024

Related Experiment Video

Updated: Jul 4, 2026

A Strategy to Validate the Role of Callose-mediated Plasmodesmal Gating in the Tropic Response
12:18

A Strategy to Validate the Role of Callose-mediated Plasmodesmal Gating in the Tropic Response

Published on: April 17, 2016

The APT1-NACsa3-GGP1 Module Enhances Salt Tolerance and Regulates Ascorbic Acid Biosynthesis in Medicago.

Jiale Wu1, Jinling Liu1, Qinyi Ye1

  • 1College of Biological Sciences, China Agricultural University, Beijing, China.

Plant, Cell & Environment
|July 3, 2026
PubMed
Summary

Researchers identified a new gene, MtNACsa3, crucial for improving salt tolerance in alfalfa. This discovery enhances understanding of plant stress responses and aids in breeding salt-resistant, nutrient-rich crops.

Keywords:
Medicago truncatulaNAC transcription factoralfalfasalt stress

More Related Videos

Production of Arbuscular Mycorrhizal (AM) Fungal Inoculum and Phenotypic Evaluation of Rice and AM Symbiosis Under Saline Conditions
07:43

Production of Arbuscular Mycorrhizal (AM) Fungal Inoculum and Phenotypic Evaluation of Rice and AM Symbiosis Under Saline Conditions

Published on: March 14, 2025

Related Experiment Videos

Last Updated: Jul 4, 2026

A Strategy to Validate the Role of Callose-mediated Plasmodesmal Gating in the Tropic Response
12:18

A Strategy to Validate the Role of Callose-mediated Plasmodesmal Gating in the Tropic Response

Published on: April 17, 2016

Production of Arbuscular Mycorrhizal (AM) Fungal Inoculum and Phenotypic Evaluation of Rice and AM Symbiosis Under Saline Conditions
07:43

Production of Arbuscular Mycorrhizal (AM) Fungal Inoculum and Phenotypic Evaluation of Rice and AM Symbiosis Under Saline Conditions

Published on: March 14, 2025

Area of Science:

  • Plant Biology
  • Molecular Genetics
  • Agricultural Science

Background:

  • Alfalfa's salt tolerance mechanisms are poorly understood, hindering breeding efforts.
  • Developing salt-tolerant crops is vital for food security in saline environments.

Purpose of the Study:

  • To identify and characterize genes involved in alfalfa's salt tolerance.
  • To elucidate the molecular mechanism of salt tolerance mediated by a novel transcription factor.

Main Methods:

  • Phenotypic analysis of mutant and overexpression lines.
  • Investigating protein localization and post-translational modifications (de-S-acylation).
  • Gene expression analysis and biochemical assays for ascorbic acid synthesis.

Main Results:

  • MtNACsa3 positively regulates salt tolerance in Medicago truncatula.
  • MtNACsa3 nuclear translocation is mediated by MtAPT1 de-S-acylation under stress.
  • MtNACsa3 up-regulates MtGGP1, boosting ascorbic acid synthesis to combat oxidative stress.

Conclusions:

  • The APT1-NACsa3-GGP1 pathway is a key mechanism for salt tolerance in alfalfa.
  • Transgenic alfalfa overexpressing MtNACsa3 exhibits enhanced salt tolerance, ascorbic acid content, and biomass.
  • This study provides valuable genetic resources for breeding salt-tolerant and AsA-rich alfalfa.