Exploring Antimicrobial Activities of Fixed Oil From Sanguisorba minor Seeds by In Vitro and In Silico Analysis

This study aimed to demonstrate in vitro and in silico antimicrobial efficacy of the fixed oil derived from the seeds of Sanguisorba minor Scop., a plant traditionally utilized for wound healing, burn treatment, and hemorrhage control. As a result of GC-MS analysis, 14 distinct saturated and unsaturated fatty acids, predominantly linoleic acid (11, 43.25%), α-linoleic acid (12, 25.33%), and oleic acid (10, 20.73%) were determined. The DPPH• and ABTS⁺ radical scavenging effects of the oil were determined to be of low-middle activity. As a result of antibacterial and antifungal activities, MIC values against tested Gram-positive and Gram-negative species ranged between 1.406 and 11.25 µL/mL. While the MBC values of plant fixed oil for bacterial species ranged between 22.5 and 45 µL/mL, this value varied between 5.625 and 11.25 µL/mL for Candida species. Molecular docking studies revealed that the antifungal and antimicrobial activities of our ligands are expected to occur at micromolar levels. In particular, the antimicrobial activity of the fixed oil was demonstrated by the higher docking scores and inhibitory effects at lower concentrations of oleic acid (10), linoleic acid (11), and α-linolenic acid (12), which specifically played a role in this process.