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Rapid Identification of Pathogens01:25

Rapid Identification of Pathogens

MALDI-TOF MS has transformed clinical microbiology by offering a rapid and reliable method for pathogen identification. The traditional approach to microbial identification typically involves time-consuming culture techniques and biochemical tests, which can delay the initiation of appropriate antimicrobial therapy. MALDI-TOF MS avoids these delays by using characteristic ribosomal protein mass patterns of microbial cells, enabling accurate species-level identification within minutes.Principle...

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Quantitative PCR of T7 Bacteriophage from Biopanning
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Published on: September 27, 2018

Rapid and quantitative phage susceptibility test by ramanome.

Xiao Han1,2, Xiaofu Wan3, Yang Zhou3,4

  • 1School of Chemistry and Chemical Engineering University of Jinan Jinan China.

Mlife
|July 3, 2026
PubMed
Summary

A novel Raman-based phage susceptibility test (RPST) rapidly assesses bacterial susceptibility to bacteriophage therapy. This lysis-independent method quantifies infection dynamics, enabling precise therapeutic strategies against antimicrobial resistance.

Keywords:
antimicrobial resistance (AMR)biomarker discoveryphage susceptibility test (PST)precision phage therapyramanome

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Published on: July 21, 2023

Area of Science:

  • Microbiology
  • Biotechnology
  • Molecular Diagnostics

Background:

  • Antimicrobial resistance (AMR) is a growing global health crisis, necessitating novel therapeutic strategies.
  • Bacteriophage therapy offers a targeted alternative to antibiotics but requires robust susceptibility testing.
  • Current phage susceptibility testing (PST) methods lack speed, quantification, and comprehensive assessment of infection parameters.

Purpose of the Study:

  • To develop a rapid, quantitative, and lysis-independent platform for phage susceptibility testing (PST).
  • To integrate host range, infection potency, and effective multiplicity of infection (MOI) determination into a single workflow.
  • To establish a dynamic framework for assessing bacteriophage efficacy relevant to clinical applications.

Main Methods:

  • Development of a ramanome-based phage susceptibility test (RPST) utilizing bacterial macromolecular composition.
  • Integration of four Raman biomarkers into a Composite Infection Index (CII) for infection assessment.
  • Analysis of CII dynamics across varying MOI and time to determine infection parameters.

Main Results:

  • RPST achieved ~1-hour results with 96.0% concordance to plaque assays.
  • The Composite Infection Index (CII) provides a continuous, population-level metric of bacterial infection.
  • RPST successfully determined the minimal effective multiplicity of infection (MOI) for therapeutic feasibility.

Conclusions:

  • RPST offers a unified, rapid, and quantitative solution for bacteriophage susceptibility testing.
  • This platform transforms PST from static assays into a dynamic assessment of phage infectivity and infection dynamics.
  • RPST facilitates the clinical translation of bacteriophage therapy by providing crucial quantitative data.