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Related Experiment Video

Updated: Jul 4, 2026

Semi-Automated Isolation of the Stromal Vascular Fraction from Murine White Adipose Tissue Using a Tissue Dissociator

Semi-Automated Isolation of the Stromal Vascular Fraction from Murine White Adipose Tissue Using a Tissue Dissociator

Published on: May 19, 2023

Adipocyte Pten Inhibition Improves Metabolic Health Associated with Expanded Lipid Storage Capacity and Reduced

Yumei Zhou¹, Yubo Wang¹, Jessica E Meerson¹

¹Department of Animal Sciences, University of Florida, Gainesville, FL 32611, USA.

Biorxiv : the Preprint Server for Biology

|July 3, 2026

Summary

Adipocyte-specific PTEN loss in mice promotes healthy adipose tissue expansion. This enhances lipid storage and insulin sensitivity, reducing inflammation and metabolic dysfunction even with obesity.

Keywords:

Pten adipocyte hypertrophy extracellular matrix insulin sensitivity lipid storage metabolic health

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Last Updated: Jul 4, 2026

Semi-Automated Isolation of the Stromal Vascular Fraction from Murine White Adipose Tissue Using a Tissue Dissociator

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Published on: May 19, 2023

An Adipocyte Cell Culture Model to Study the Impact of Protein and Micro-RNA Modulation on Adipocyte Function

An Adipocyte Cell Culture Model to Study the Impact of Protein and Micro-RNA Modulation on Adipocyte Function

Published on: May 4, 2021

Area of Science:

Metabolic Diseases
Obesity Research
Adipose Tissue Biology

Background:

Adipose tissue dysfunction is a key driver of obesity-associated insulin resistance.
The role of expanded adipocyte lipid storage in metabolic health is not fully understood.

Purpose of the Study:

To investigate the effects of chronic PTEN loss in adipocytes on adipose tissue remodeling and systemic metabolism.
To determine if enhanced lipid storage in adipocytes can lead to improved metabolic health.

Main Methods:

Generated adipocyte-specific PTEN knockout mice (PTEN AKO) using Adipoq-Cre.
Analyzed adipose tissue remodeling, systemic metabolism, and insulin sensitivity under chow and high-fat diet conditions.
Performed transcriptomic analysis of inguinal white adipose tissue.

Main Results:

PTEN AKO mice showed increased adiposity and adipocyte hypertrophy but lower blood glucose and insulin levels.
Enhanced insulin sensitivity and reduced hepatic lipid accumulation were observed without systemic metabolic deterioration.
PTEN deficiency preserved brown adipose tissue function (UCP1, OXPHOS) and promoted pathways for lipid metabolism and reduced inflammation in white adipose tissue.

Conclusions:

Adipocyte PTEN deficiency promotes metabolically healthy adipose expansion.
Enhanced lipid storage capacity, preserved adipocyte function, and reduced inflammation contribute to improved metabolic health.
PTEN loss induces coordinated structural adaptations in adipose tissue to support adipocyte expansion.