A curated list of InDel markers for rapid genetic mapping in Arabidopsis thaliana

Identification of novel functional genes through mutant analysis is one of the most powerful approaches in plant science. In the model plant Arabidopsis thaliana, this approach typically combines whole-genome sequencing of the mutant with fine mapping using molecular polymorphic markers between two ecotypes to narrow down the genomic region containing the mutant allele. Among PCR-based mapping methods, insertion/deletion (InDel) markers allow for precise and rapid genotyping with relatively simple technology. In this report, we present a set of 49 new InDel markers distributed across the entire genome between Columbia (Col-0) and Landsberg erecta (Ler) accessions, specifically suitable for initial mapping experiments. These markers are designed to detect insertions in Col-0 or deletions in Ler with a length polymorphism greater than 100 bp and will help facilitate map-based gene cloning.