High-Load Amino Acid Nanoemulsion: Physicochemical Optimization and Dermal Bioactivity
Pornnapa Thanomrak1, Kittipong Sanookpan1, Sirikool Thamnium1
1Department of Pharmaceutics and Industrial Pharmacy, Faculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok 10330, Thailand.
ACS Omega
|July 3, 2026
Summary
This study developed stable nanoemulsions delivering high amino acid loads for skin repair. Optimized formulations enhanced collagen and elastin production, showing potential for improved dermal delivery and extracellular matrix renewal.
Area of Science:
- Biotechnology
- Dermatology
- Cosmetic Science
Background:
- Amino acids like GABA, glycine, and proline are vital for extracellular matrix renewal.
- Their high hydrophilicity hinders effective delivery into the dermis.
- Developing suitable delivery systems is crucial for harnessing their benefits.
Purpose of the Study:
- To create a stable oil-in-water nanoemulsion for high amino acid loading (20% w/w).
- To assess the physicochemical properties, stability, and biological activity of the developed nanoemulsions.
- To optimize the formulation and processing for effective dermal delivery.
Main Methods:
- Nanoemulsions prepared via high-pressure homogenization.
- Varied surfactant concentrations (5-7% w/w) and pressures (80-120 MPa).
- Evaluated physicochemical properties, stability, amino acid retention, and biological activity in fibroblasts.
Main Results:
- A formulation with 5% surfactant and 120 MPa showed optimal stability and biological response.
- The nanoemulsion retained over 95% of actives for 12 weeks.
- Significantly enhanced collagen and elastin gene expression in fibroblasts.
Conclusions:
- Process optimization yields stable, high-load amino acid nanoemulsions.
- These nanoemulsions demonstrate potential for enhanced dermal delivery.
- The technology offers promising applications in skin repair and extracellular matrix renewal.
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