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Related Concept Videos

Drug Dosing in Renal Diseases: Measurement of Glomerular Filtration Rate01:25

Drug Dosing in Renal Diseases: Measurement of Glomerular Filtration Rate

The glomerular filtration rate (GFR) is a critical indicator of kidney health, reflecting how well the kidneys filter blood. Changes in GFR can signal potential kidney impairment, necessitating accurate measurement methods to monitor kidney function effectively.Various molecules can serve as markers for GFR measurement, with the ideal marker meeting several specific criteria. It must freely filter at the glomerulus, avoid reabsorption or secretion by the renal tubules, remain unmetabolized, not...
Drug Dosing in Renal Diseases: Estimation of Glomerular Filtration Rate Based on Serum Creatinine Concentration01:28

Drug Dosing in Renal Diseases: Estimation of Glomerular Filtration Rate Based on Serum Creatinine Concentration

Glomerular filtration rate (GFR) can be estimated from serum creatinine using the modification of diet in renal disease (MDRD) formula or the chronic kidney disease–epidemiology collaboration (CKD–EPI) equation. Both methods are widely used in clinical practice to assess kidney function and guide treatment decisions.The MDRD equation does not require weight or height measurements and is normalized to the body surface area of 1.73 m², considered the average adult surface area. This equation is...
Renal Clearance01:23

Renal Clearance

The glomerular filtration rate (GFR) is a critical marker of kidney function, reflecting the efficiency of filtration by the glomeruli. Renal clearance of specific substances, such as inulin or creatinine, is commonly used to measure GFR.
Renal clearance refers to the volume of plasma cleared of a specific substance, such as creatinine, per unit of time. To measure clearance, urine samples are collected over a 24-hour period during each bladder voiding, followed by a single blood sample at the...
Drug Dosing in Renal Diseases: Measurement of Serum Creatinine Concentration and Clearance01:25

Drug Dosing in Renal Diseases: Measurement of Serum Creatinine Concentration and Clearance

In healthy individuals, serum creatinine levels remain stable due to a balance between its constant production—primarily from muscle metabolism—and renal excretion. Creatinine is freely filtered by the glomeruli, making it a valuable marker for estimating renal function. When the glomerular filtration rate (GFR) decreases, the kidneys can only eliminate less creatinine, causing serum levels to rise.Serum creatinine concentration is widely used to estimate creatinine clearance (Clcr), a...
Factors Affecting Renal Clearance: Renal Impairment01:17

Factors Affecting Renal Clearance: Renal Impairment

Renal dysfunction significantly impairs the renal clearance of drugs, leading to potential complications in drug therapy. Renal failure, which can be caused by various factors, poses a significant challenge in the elimination of drugs from the body.
One condition associated with renal failure is uremia. Uremia is characterized by impaired glomerular filtration and fluid accumulation in the body. This condition hinders the renal clearance of drugs, resulting in drug accumulation and potential...
Serum Studies: Renal Function Tests01:24

Serum Studies: Renal Function Tests

Renal function tests are crucial for assessing kidney health, monitoring disease progression, and evaluating the kidneys' efficiency in waste elimination, fluid balance, and electrolyte regulation. These tests offer critical insights into kidney function, even though routine measurements may appear normal until there is a significant decline in the glomerular filtration rate or GFR. Typically, signs of kidney impairment only become evident when the GFR falls to about 50% of its normal level.

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Related Experiment Video

Updated: Jul 4, 2026

Transcutaneous Assessment of Renal Function in Conscious Rodents
07:18

Transcutaneous Assessment of Renal Function in Conscious Rodents

Published on: March 26, 2016

Reliable quantification of renal function from frozen blood samples.

Scott R French, Gavin C Culwell, Haley E Wiskoski

    Medrxiv : the Preprint Server for Health Sciences
    |July 3, 2026
    PubMed
    Summary

    Estimated glomerular filtration rate (eGFR) can be reliably measured from frozen blood samples, showing high agreement with point-of-care tests. This finding is crucial for accurately interpreting Alzheimer's disease (AD) blood biomarkers in aging populations.

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    A High-throughput Method for Measurement of Glomerular Filtration Rate in Conscious Mice
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    A High-throughput Method for Measurement of Glomerular Filtration Rate in Conscious Mice

    Published on: May 10, 2013

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    Last Updated: Jul 4, 2026

    Transcutaneous Assessment of Renal Function in Conscious Rodents
    07:18

    Transcutaneous Assessment of Renal Function in Conscious Rodents

    Published on: March 26, 2016

    A High-throughput Method for Measurement of Glomerular Filtration Rate in Conscious Mice
    07:07

    A High-throughput Method for Measurement of Glomerular Filtration Rate in Conscious Mice

    Published on: May 10, 2013

    Area of Science:

    • Gerontology
    • Nephrology
    • Neurology

    Background:

    • Renal function influences Alzheimer's disease (AD) blood biomarker levels independently of AD pathology.
    • Quantifying estimated glomerular filtration rate (eGFR) from frozen samples could address unaccounted renal function in AD biomarker studies.
    • The reliability of eGFR assessment from long-term frozen blood samples was previously unestablished.

    Purpose of the Study:

    • To determine the validity of eGFR evaluation from long-term frozen serum and plasma blood samples.
    • To assess the agreement between eGFR measurements from point-of-care whole blood (POC-WB) and frozen blood samples.

    Main Methods:

    • Adults aged 50-85 with vascular risk factors were recruited.
    • Creatinine was assessed using iSTAT (Abbott) in POC-WB and frozen serum/plasma samples.
    • eGFR was calculated using the 2021 CKD-EPI equation; agreement was assessed using Cohen's kappa.

    Main Results:

    • 134 participants had POC-WB eGFR data; 23.1% had chronic kidney disease.
    • Frozen serum and plasma samples showed strong agreement with POC-WB for eGFR (Kw=0.90-0.95, P<0.001).
    • Pre-analytical factors minimally impacted eGFR differences between POC-WB and frozen samples.

    Conclusions:

    • Renal function assessment from frozen blood samples is highly consistent with POC-WB measurements.
    • This method is relevant for interpreting AD blood biomarkers in aging and vascular populations with potential renal impairment.