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Flow Cytometry

Flow Cytometry

The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
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Related Experiment Video

Updated: Jul 4, 2026

Asymmetrical Flow Field-Flow Fractionation for Sizing of Gold Nanoparticles in Suspension

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Published on: September 11, 2020

A Rapid Method for Accurately Determining Lipid Nanoparticle Size Using Nano-Flow Cytometry.

Brittany Rein¹, Christiano Marconi², Daniil Shabashvilli¹

¹ICBR University of Florida.

Journal of Biomolecular Techniques : JBT

|July 3, 2026

Summary

This summary is machine-generated.

Lipid nanoparticles (LNPs) are crucial for drug delivery. This study found that nano-flow cytometry is a highly accurate method for sizing these nanoparticles, aligning well with electron microscopy.

Keywords:

CryoTEM lipid nanoparticles nano-flow cytometry

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Last Updated: Jul 4, 2026

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Published on: March 28, 2014

Area of Science:

Biotechnology
Nanotechnology
Pharmaceutical Sciences

Background:

Lipid nanoparticles (LNPs) are essential carriers for nucleic acid-based therapeutics, protecting payloads and enhancing delivery.
Assessing LNP size distribution and loading efficiency is critical for formulation quality control.
Various techniques exist for nanoparticle sizing, each with unique advantages and limitations.

Purpose of the Study:

To evaluate and compare different methods for determining LNP size distribution.
To assess the accuracy and robustness of nano-flow cytometry for LNP sizing.
To compare nano-flow cytometry results with conventional cryogenic transmission electron microscopy.

Main Methods:

Nanoparticle tracking analysis (NTA)
Dynamic light scattering (DLS)
Two nano-flow cytometry instruments (NanoFCM, CytoFLEX nano)
Cryogenic transmission electron microscopy (Cryo-TEM) for reference

Main Results:

Nano-flow cytometry instruments provided robust and accurate LNP size measurements.
Results from nano-flow cytometry showed high concordance with Cryo-TEM measurements.
Compared to NTA and DLS, nano-flow cytometry offered superior accuracy for LNP sizing.

Conclusions:

Nano-flow cytometry is a reliable and accurate technique for sizing lipid nanoparticles.
This method offers a valuable alternative for quality control in nucleic acid-based therapeutic development.
The findings support the adoption of nano-flow cytometry for LNP characterization.