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Related Experiment Video

Updated: Jul 4, 2026

Synthesizing Lipid Nanoparticles by Turbulent Flow in Confined Impinging Jet Mixers
08:10

Synthesizing Lipid Nanoparticles by Turbulent Flow in Confined Impinging Jet Mixers

Published on: August 23, 2024

3D-Printed Milli-Fluidic Mixing-Extrusion Platform for Continuous, High-Throughput LNP Production.

Leekang Jeon1, Seeun Kim1, Hanjin Seo1

  • 1Department of Chemical Engineering, Pohang University of Science and Technology (POSTECH), Pohang, Gyeongbuk, South Korea.

Small (Weinheim an Der Bergstrasse, Germany)
|July 3, 2026
PubMed
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This study introduces a novel hybrid platform for manufacturing lipid nanoparticles (LNPs), overcoming scalability and cost issues. The reusable milli-fluidic system achieves precise, continuous LNP production, enhancing therapeutic delivery.

Area of Science:

  • Biotechnology
  • Materials Science
  • Chemical Engineering

Background:

  • Lipid nanoparticles (LNPs) are crucial for delivering mRNA vaccines and genetic medicines.
  • Current LNP manufacturing methods face challenges in balancing precision, scalability, and cost.
  • Microfluidics offers particle control but suffers from fouling and expensive disposable chips, hindering reproducible production.

Purpose of the Study:

  • To develop a scalable, cost-effective, and contamination-resistant platform for LNP manufacturing.
  • To address the limitations of existing microfluidic and bulk LNP synthesis methods.
  • To improve the reproducibility and efficiency of industrial LNP production.

Main Methods:

  • Integration of a reusable 3D-printed milli-fluidic mixer with inline membrane extrusion.
Keywords:
3D‐printingextrusionhigh‐throughputlipid nanoparticlesmixing

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Formulating and Characterizing Lipid Nanoparticles for Gene Delivery using a Microfluidic Mixing Platform
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Formulating and Characterizing Lipid Nanoparticles for Gene Delivery using a Microfluidic Mixing Platform

Published on: February 25, 2021

Related Experiment Videos

Last Updated: Jul 4, 2026

Synthesizing Lipid Nanoparticles by Turbulent Flow in Confined Impinging Jet Mixers
08:10

Synthesizing Lipid Nanoparticles by Turbulent Flow in Confined Impinging Jet Mixers

Published on: August 23, 2024

Formulating and Characterizing Lipid Nanoparticles for Gene Delivery using a Microfluidic Mixing Platform
09:41

Formulating and Characterizing Lipid Nanoparticles for Gene Delivery using a Microfluidic Mixing Platform

Published on: February 25, 2021

  • Engineering a robust 3D internal architecture at the milli-scale for rapid mixing and reduced clogging.
  • Continuous synthesis enabling single-pass refinement of LNPs.

    • Main Results:

    • Achieved rapid mixing and mitigated clogging risks compared to micro-channels.
    • Demonstrated enhanced process reproducibility with a durable, easy-to-clean system.
    • Produced high-quality, uniform LNPs at flow rates up to 20 mL/min.

    Conclusions:

    • The hybrid continuous synthesis platform offers a scalable and cost-effective pathway for industrial LNP manufacturing.
    • This system overcomes the limitations of disposable cartridge-based microfluidic systems.
    • The developed technology streamlines LNP production, improving accessibility for therapeutic applications.