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Structural and evolutionary divergence of the RAG1/1L N-terminal zinc-coordinating domain
Junye Hong1, Zhijun Liu2, Wei Xu1
1School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China.
None:
RAG1/2 catalyzes V(D)J recombination to assemble antigen receptor genes, but the RAG1 N-terminal zinc-coordinating domain (NZD) has remained structurally and functionally uncharacterized. Here, we determine the NMR structure of mouse RAG1 NZD, revealing a compact, zinc-dependent fold composed of four α-helices and two short β-strands. This architecture is organized into two interdigitated zinc-coordinating modules, ZMa and ZMb. Structural similarity searches identify no close homolog with the same overall architecture, suggesting that NZD represents a previously undescribed zinc-coordinating fold. Comparative analyses show that NZD is broadly conserved across RAG1 and RAG1-like proteins, while also displaying lineage-specific remodeling, including acquisition of the H2 helix in jawed vertebrates. Guided by structure prediction, we further identify putative NZD-like domains in Chapaev transposases, supporting a possible evolutionary link between RAG1/RAG1L NZDs and Chapa domains. Together, these findings provide a structural framework for mechanistic and evolutionary analyses of RAG1.
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