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Related Concept Videos

Karyotyping01:17

Karyotyping

Describing the number and physical features of chromosomes can reveal abnormalities that underlie genetic diseases. This description is facilitated by special staining techniques that produce a particular banding pattern on each chromosome. State-of-the-art techniques make this approach even more powerful, enabling the detection of individual genes that cause disease.A Simple Chromosome Staining Technique Provides Valuable Scientific InsightSome genetic diseases can be detected by looking at...
Karyotyping01:17

Karyotyping

Describing the number and physical features of chromosomes can reveal abnormalities that underlie genetic diseases. This description is facilitated by special staining techniques that produce a particular banding pattern on each chromosome. State-of-the-art techniques make this approach even more powerful, enabling the detection of individual genes that cause disease.A Simple Chromosome Staining Technique Provides Valuable Scientific InsightSome genetic diseases can be detected by looking at...
Genetic Screens02:46

Genetic Screens

Genetic screens are tools used to identify genes and mutations responsible for phenotypes of interest. Genetic screens help identify individuals or a group of people at risk of developing  genetic diseases and help them with early intervention, targeted therapy, and reproductive options.
Forward genetic screens
Forward or “classical” genetic screens involve creating random mutations in an organism’s DNA using radiation, mutagens, or insertion of additional bases, which result in visible changes...
Trihybrid Crosses02:27

Trihybrid Crosses

Trihybrid Crosses
Some of Mendel’s crosses examined three pairs of contrasting characteristics. Such a cross is called a trihybrid cross. A trihybrid cross is a combination of three individual monohybrid crosses. For example, plant height (tall vs. short), seed shape (round vs. wrinkled), and seed color (yellow vs. green).
The F1 generation plants of a trihybrid cross are heterozygous for all three traits and produce eight gametes. Upon self-fertilization, these gametes have an equal chance to...

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Related Experiment Video

Updated: Jul 5, 2026

High Throughput Yeast Strain Phenotyping with Droplet-Based RNA Sequencing
07:55

High Throughput Yeast Strain Phenotyping with Droplet-Based RNA Sequencing

Published on: May 21, 2020

CYClones: A highly powered, fully genotyped, 8-parent yeast mapping population.

Gareth A Cromie1, Russell S Lo1, Trey S Morgan1

  • 1Pacific Northwest Research Institute, Seattle, Washington 98122, USA.

G3 (Bethesda, Md.)
|July 3, 2026
PubMed
Summary

Researchers created CYClones, a large library of yeast segregants, to study genetic diversity and complex traits. This resource aids in dissecting the genetic basis of variations in Saccharomyces cerevisiae.

Keywords:
Budding yeastFunnel CrossMapping populationMulti-parent advanced generation intercross

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Last Updated: Jul 5, 2026

High Throughput Yeast Strain Phenotyping with Droplet-Based RNA Sequencing
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Published on: May 21, 2020

The Green Monster Process for the Generation of Yeast Strains Carrying Multiple Gene Deletions
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Published on: December 15, 2012

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Area of Science:

  • Genetics
  • Yeast Biology
  • Population Genetics

Background:

  • Saccharomyces cerevisiae exhibits significant genetic diversity across natural isolates.
  • Understanding this diversity is key to dissecting complex traits.
  • Previous resources have not fully captured the global genetic variation in yeast.

Purpose of the Study:

  • To construct and characterize CYClones, a comprehensive library of yeast segregants.
  • To enable high-resolution genetic dissection of complex traits in Saccharomyces cerevisiae.
  • To provide a community resource for exploring yeast genetic architecture.

Main Methods:

  • Generated 11,392 segregants from a multiparental cross of eight diverse S. cerevisiae strains.
  • Imputed whole-genome sequences for all segregants.
  • Characterized haplotype representation and genetic diversity captured by the library.

Main Results:

  • CYClones captures a substantial fraction of global S. cerevisiae genetic diversity.
  • Haplotype representation is well-maintained across the genome.
  • The library provides high power (≥95%) for detecting variants with low heritability and fine mapping resolution.

Conclusions:

  • CYClones is a powerful resource for dissecting the genetic architecture of complex and quantitative traits in yeast.
  • It facilitates the identification of causal variants underlying phenotypic diversity.
  • This resource supports research into context-dependent mutational effects in Saccharomyces cerevisiae.