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Updated: Jul 5, 2026

Extraction of Lignin with High β-O-4 Content by Mild Ethanol Extraction and Its Effect on the Depolymerization Yield
Published on: January 7, 2019
Structural Regulation of Lignin Using an Al3+/Zn2+ Bimetallic Lewis Acid System to Enhance Enzymatic Saccharification
Xiliang Wu1, Guanghui Zhu1, Yang Yang1
1Guangdong Laboratory for Lingnan Modern Agriculture, Guangdong Engineering Technology Research Center of Agricultural and Forestry Biomass, Key Laboratory of Energy Plants Resource and Utilization, Ministry of Agriculture and Rural Affairs, College of Future Biomass, South China Agricultural University, Guangzhou 510642, PR China.
None:
This study employed AlCl3/ZnCl2 bimetallic salt catalysis with ethanol organic solvent for sugar cane bagasse pretreatment. The process retained 80.12% cellulose while removing ∼70% xylan and 80.56% lignin, achieving 90.18% glucose conversion during enzymatic hydrolysis. Comprehensive characterization and DFT calculations revealed a synergistic dual-action mechanism: strong Lewis acid Al3+ extensively cleaved β-O-4 bonds (reducing them to 40.68%), whereas weak Lewis acid Zn2+ interacted broadly with side chains and aromatic rings. This synergy facilitated substantial lignin removal and minimized total phenolic OH content in residual lignin (1.32 mmol/g), thereby suppressing unproductive cellulase adsorption. Additionally, the bimetallic system exhibited stronger overall interactions with the model compound GG compared to single-component systems. This integrated approach establishes a robust foundation for efficient lignocellulose fractionation and downstream bioconversion.
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