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A new alkaloid isolated from Portulaca oleracea L. and its anti-inflammatory mechanism
Pengyan Liu1, Jing Liu1, Yingdai Zhao1
1School of Pharmacy, Liaoning University of Traditional Chinese Medicine, Dalian, P.R. China.
Abstract:
A alkaloid identified as 8-(2-hydroxyethyl)-3,8-diazabicyclo[4.2.0]octa-1,4,6-triene-2-carbaldehyde named Oleradiazahyde was isolated. Its skeleton was assigned by UV, IR, NMR, as well as UHPLC-ESI-Q-TOF/MS. Simultaneously, LPS-activated RAW 264.7 tests revealed that 15 μM compound suppressed NO/IL-6, and 5 μM restrained IL-1β secretion. At 15 μM, oleradiazahyde greatly reduced iNOS protein as well as iNOS and COX-2 mRNA. This compound also restrained COX-2 protein production when administered at 5 μM.
