Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A Transgenic Mouse Model to Track MRC1-High Macrophages Using In Vivo Optical Imaging.

International journal of molecular sciences·2026
Same author

CTG-Initiated Cryptic Peptide Translation Up- and Downstream of a Canonical ATG Start Codon Is Enhanced by TLR Stimulation and Induces Tumor Regression in Mice.

Cancer immunology research·2025
Same author

Novel <i>STAT3</i> Y360C Gain-of-function Variant Underlies Immune Dysregulation and Aberrancy in Mitochondrial Dynamics.

Immune network·2025
Same author

Point-of-care testing reduces antibiotic prescribing in acute exacerbations of chronic obstructive pulmonary disease: A systematic review and meta-analysis.

International journal of infectious diseases : IJID : official publication of the International Society for Infectious Diseases·2025
Same author

Bryostatin-1 enhances the proliferation and functionality of exhausted CD8+ T cells by upregulating MAP Kinase 11.

Frontiers in immunology·2025
Same author

Pulmonary artery choriocarcinoma mimicking pulmonary thromboembolism: a case report.

Translational cancer research·2025

Related Experiment Video

Updated: Jul 5, 2026

A Preclinical Mouse Model of Osteosarcoma to Define the Extracellular Vesicle-mediated Communication Between Tumor and Mesenchymal Stem Cells
11:15

A Preclinical Mouse Model of Osteosarcoma to Define the Extracellular Vesicle-mediated Communication Between Tumor and Mesenchymal Stem Cells

Published on: May 6, 2018

IL-18 derived from microwave-ablated osteosarcoma facilitates antitumor response.

Jielong Zhou1, Yunhui Zhang1, Wenjie Yang2

  • 1School of Medicine, South China University of Technology, Guangzhou, Guangdong, China.

Journal for Immunotherapy of Cancer
|July 3, 2026
PubMed
Summary

Microwave ablation (MWA) boosts interleukin-18 (IL-18) and enhances immune cell function in osteosarcoma (OSA). Combining IL-18 with anti-PD-1 therapy offers a promising treatment strategy for OSA.

Keywords:
Bone CancerCytokineDendriticImmune modulatoryTumor microenvironment - TME

More Related Videos

Cytotoxic Efficacy of Photodynamic Therapy in Osteosarcoma Cells In Vitro
08:04

Cytotoxic Efficacy of Photodynamic Therapy in Osteosarcoma Cells In Vitro

Published on: March 18, 2014

Improved Visualization of Lung Metastases at Single Cell Resolution in Mice by Combined In-situ Perfusion of Lung Tissue and X-Gal Staining of lacZ-Tagged Tumor Cells
08:47

Improved Visualization of Lung Metastases at Single Cell Resolution in Mice by Combined In-situ Perfusion of Lung Tissue and X-Gal Staining of lacZ-Tagged Tumor Cells

Published on: August 21, 2012

Related Experiment Videos

Last Updated: Jul 5, 2026

A Preclinical Mouse Model of Osteosarcoma to Define the Extracellular Vesicle-mediated Communication Between Tumor and Mesenchymal Stem Cells
11:15

A Preclinical Mouse Model of Osteosarcoma to Define the Extracellular Vesicle-mediated Communication Between Tumor and Mesenchymal Stem Cells

Published on: May 6, 2018

Cytotoxic Efficacy of Photodynamic Therapy in Osteosarcoma Cells In Vitro
08:04

Cytotoxic Efficacy of Photodynamic Therapy in Osteosarcoma Cells In Vitro

Published on: March 18, 2014

Improved Visualization of Lung Metastases at Single Cell Resolution in Mice by Combined In-situ Perfusion of Lung Tissue and X-Gal Staining of lacZ-Tagged Tumor Cells
08:47

Improved Visualization of Lung Metastases at Single Cell Resolution in Mice by Combined In-situ Perfusion of Lung Tissue and X-Gal Staining of lacZ-Tagged Tumor Cells

Published on: August 21, 2012

Area of Science:

  • Immunology
  • Oncology
  • Biomedical Engineering

Background:

  • Osteosarcoma (OSA) is a primary bone cancer in children with stagnant survival rates.
  • Microwave ablation (MWA) is an emerging thermal therapy for bone tumors.
  • MWA's mechanism for inducing antitumor immunity, particularly its role in interleukin-18 (IL-18) expression and dendritic cell (DC) function, requires elucidation.

Purpose of the Study:

  • To investigate the intrinsic mechanisms by which MWA induces antitumor immunity in osteosarcoma.
  • To explore the role of IL-18 in MWA-mediated immune activation.
  • To evaluate the therapeutic potential of combining IL-18 with anti-programmed cell death protein 1 (PD-1) therapy for osteosarcoma.

Main Methods:

  • In vitro co-culture of MWA-treated osteosarcoma with bone marrow-derived dendritic cells (BMDCs).
  • RNA sequencing to analyze molecular changes in BMDCs stimulated by MWA-treated osteosarcoma.
  • In vivo mouse models of osteosarcoma to assess immune responses and therapeutic efficacy of IL-18 and anti-PD-1 combination therapy.

Main Results:

  • MWA treatment enhanced BMDC activation, antigen uptake, and cross-presentation.
  • MWA increased CD8+ T cell and CD11c+ DC infiltration in tumors and draining lymph nodes.
  • Tumor suppression was IL-18 dependent, mediated by IL-18 release from MWA-treated osteosarcoma and interaction with IL-18 receptors on DCs; combination therapy showed additive effects.

Conclusions:

  • IL-18-mediated signaling plays a critical role in MWA-induced protective immune responses against osteosarcoma.
  • This study provides a novel mechanism for MWA-induced immune activation.
  • IL-18 cytokine therapy represents a feasible and effective potential treatment approach for osteosarcoma.