Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Jul 5, 2026

Point-of-care CRISPR-based Diagnostics with Premixed and Freeze-dried Reagents
10:16

Point-of-care CRISPR-based Diagnostics with Premixed and Freeze-dried Reagents

Published on: August 16, 2024

DNAzyme-Enhanced CRISPR/Cas12a Cascade Enables Isothermal, One-Pot RNA Diagnostics.

Ruyue Wei1, Shuqi Wang1, Yufan Li1

  • 1College of Chemistry, Chemical Engineering and Materials Science, Key Laboratory of Molecular and Nano Probes, Ministry of Education, Collaborative Innovation Center of Functionalized Probes for Chemical Imaging in Universities of Shandong, Institute of Molecular and Nano Science, Shandong Normal University, Jinan 250014, P. R. China.

ACS Applied Materials & Interfaces
|July 3, 2026
PubMed
Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Nano-radiosensitizers targeting tumor radioresistance: mechanisms, rational design, and clinical prospects.

Chemical communications (Cambridge, England)·2026
Same author

Organoids, organ-on-a-chip, and microtumors: Biomimetic 3D tumor models advancing drug development and precision medicine.

Acta pharmaceutica Sinica. B·2026
Same author

Beyond the Hazard Ratio: Time-Varying Treatment Effects in Genitourinary Cancer Trials.

Clinical genitourinary cancer·2026
Same author

Association between frailty and major chronic disease-free life expectancy: a large community-based longitudinal study.

Postgraduate medicine·2026
Same author

Intranasal fluorinated nano-adjuvant orchestrates CCL2-driven pre-DC recruitment and transmucosal antigen delivery to elicit long-term immunity against influenza.

Journal of controlled release : official journal of the Controlled Release Society·2026
Same author

Effectiveness and mechanisms of isometric resistance exercise to reduce blood pressure in a Chinese population: study protocol for a randomized-controlled trial.

Trials·2026
Same journal

Ti/Sr Gradient Doping with SrTiO<sub>3</sub> Coating for Mitigating Strain and Oxygen Loss in Ni-Rich Cathode.

ACS applied materials & interfaces·2026
Same journal

Metallic Lead to Perfect Perovskite: A Bottom-Up Vapor-Assisted Colloidal Strategy for High-Performance Solar Cells.

ACS applied materials & interfaces·2026
Same journal

Two-Dimensional VSe<sub>2</sub>@Polypyrrole Heterostructure Enables Stable High-Rate Lithium-Sulfur Batteries.

ACS applied materials & interfaces·2026
Same journal

A Multifunctional Hydrogel Integrating Hemostatic, Antioxidant, and Antibacterial Properties for Infected and Diabetic Wound Regeneration.

ACS applied materials & interfaces·2026
Same journal

Tunable Interfacial to Filamentary Resistive Switching Mechanism in Room-Temperature-Grown Amorphous YBa<sub>2</sub>Cu<sub>3</sub>O<sub><i>x</i></sub> with Excess Cu Addition.

ACS applied materials & interfaces·2026
Same journal

Bioinspired Rhombic VO<sub>2</sub> Metasurface with Low Solar Absorptance for Self-adaptive All-Weather Building Thermal Management.

ACS applied materials & interfaces·2026
See all related articles
This summary is machine-generated.

This study introduces a novel DNAzyme-CRISPR/Cas12a system for sensitive RNA detection. The one-pot assay significantly enhances nucleic acid detection sensitivity through cascade amplification.

Area of Science:

  • Molecular Biology
  • Biotechnology
  • Biosensing

Background:

  • Integrating DNAzymes with CRISPR/Cas systems enhances CRISPR/Cas12a diagnostics.
  • Current methods face challenges in simplicity, specificity, and sensitivity due to complex workflows.

Purpose of the Study:

  • To develop a universal nucleic acid preamplifier using an RNA-triggered DNAzyme circuit integrated with CRISPR/Cas12a.
  • To enable simple, specific, sensitive, one-pot, and homogeneous nucleic acid detection.

Main Methods:

  • An RNA-triggered DNAzyme circuit was integrated with CRISPR/Cas12a for cascade amplification.
  • DNAzyme activity was restored upon target recognition, initiating a secondary CRISPR/Cas amplification.
  • Detection was visualized using a lateral flow assay with a reporter cleavage system.
Keywords:
CRISPR/Cas12aDNAzymeRNA detectionlateral flow assayone-pot

More Related Videos

Field-Deployable Candidatus Liberibacter asiaticus Detection Using Recombinase Polymerase Amplification Combined with CRISPR-Cas12a
09:03

Field-Deployable Candidatus Liberibacter asiaticus Detection Using Recombinase Polymerase Amplification Combined with CRISPR-Cas12a

Published on: December 23, 2022

On-site DNA Detection of Trypanosomatid Parasites and Nosema ceranae Through Alkaline Lysis Coupled to RPA/CRISPR/Cas12a System
07:46

On-site DNA Detection of Trypanosomatid Parasites and Nosema ceranae Through Alkaline Lysis Coupled to RPA/CRISPR/Cas12a System

Published on: July 18, 2025

Related Experiment Videos

Last Updated: Jul 5, 2026

Point-of-care CRISPR-based Diagnostics with Premixed and Freeze-dried Reagents
10:16

Point-of-care CRISPR-based Diagnostics with Premixed and Freeze-dried Reagents

Published on: August 16, 2024

Field-Deployable Candidatus Liberibacter asiaticus Detection Using Recombinase Polymerase Amplification Combined with CRISPR-Cas12a
09:03

Field-Deployable Candidatus Liberibacter asiaticus Detection Using Recombinase Polymerase Amplification Combined with CRISPR-Cas12a

Published on: December 23, 2022

On-site DNA Detection of Trypanosomatid Parasites and Nosema ceranae Through Alkaline Lysis Coupled to RPA/CRISPR/Cas12a System
07:46

On-site DNA Detection of Trypanosomatid Parasites and Nosema ceranae Through Alkaline Lysis Coupled to RPA/CRISPR/Cas12a System

Published on: July 18, 2025

Main Results:

  • The system achieved high sensitivity by converting a single RNA molecule into numerous initiators.
  • The DNAzyme acted as a signal amplifier, breaking the one-to-one target-Cas12a activation ratio.
  • The biosensor demonstrated programmability and universality by customizing the blocker strand.

Conclusions:

  • The integrated DNAzyme-CRISPR/Cas12a system provides efficient signal amplification in a one-pot format.
  • This approach offers a promising solution for practical applications in nucleic acid detection.
  • The system enhances sensitivity and simplifies detection workflows for molecular diagnostics.