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Updated: Jul 5, 2026

Identification of Post-translational Modifications of Plant Protein Complexes

Identification of Post-translational Modifications of Plant Protein Complexes

Published on: February 22, 2014

Late Blight Effector Pi05910 Suppresses Plant Immunity by Promoting Destabilisation of Host Scaffold Protein RACK1.

Xiong Liu¹, Yisa Wang¹, Song Liu¹

¹State Key Laboratory of Crop Stress Resistance and High-Efficiency Production and College of Agronomy, Northwest A&F University, Yangling, Shaanxi, China.

Molecular Plant Pathology

|July 4, 2026

Summary

Phytophthora infestans effector Pi05910 suppresses potato immunity by targeting the Receptor for Activated C Kinase 1 (RACK1). This interaction promotes late blight susceptibility, revealing a pathogen strategy to disarm host defenses.

Keywords:

Phytophthora infestans 26S proteasome RXLR effector Pi05910 protein translation scaffold protein RACK1 susceptibility

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Published on: October 1, 2015

Area of Science:

Plant pathology
Molecular plant-microbe interactions
Oomycete biology

Background:

Late blight, caused by Phytophthora infestans, severely impacts potato and tomato crops.
Plant immunity involves intricate signaling pathways often targeted by pathogen effectors.
R-XLR effectors from P. infestans are known virulence factors that suppress host immunity.

Purpose of the Study:

To investigate the role of the P. infestans effector Pi05910 in late blight pathogenesis.
To identify the host target of Pi05910 and elucidate its function in plant immunity.
To understand how Pi05910 manipulates host factors to promote P. infestans infection.

Main Methods:

Functional analysis of StRACK1 in potato and NbRACK1 in Nicotiana benthamiana using gene silencing and overexpression.
Investigating the interaction between Pi05910 and RACK1 using co-immunoprecipitation.
Assessing the impact of RACK1 destabilization on plant immune responses and pathogen colonization.

Main Results:

Pi05910 targets and destabilizes the potato immune scaffold protein StRACK1 via the 26S proteasome pathway.
Silencing RACK1 increased susceptibility to P. infestans, while RACK1 overexpression enhanced resistance.
RACK1 destabilization inhibited PAMP-triggered immunity, including ROS burst and defense gene expression, and suppressed plant growth.

Conclusions:

The P. infestans effector Pi05910 functions as a virulence factor by sabotaging the host immune scaffold StRACK1.
Pathogen effectors can promote susceptibility by targeting key host proteins involved in immune signaling.
This study provides novel insights into effector-triggered susceptibility mechanisms in potato late blight.